Optimization of Glycerol Production by a New Osmotolerant Wickerhamomyces anomalus AUMC 11687 Yeast Strain Using Response Surface

Abstract

Glycerol considered as a great tool for industrial applications. Mechanism of glycerol production by yeast cell become highly attractive and interesting. Many factors affect glycerol yield throughout fermentation process. For this reason an osmotolerant glycerol producer yeast strain Wickerhamomyces anomalus AUMC 11687 was selected for optimization of glycerol production conditions by using response surface methodology. Four independents variables (temperature, pH, sugar and glycerol concentration as osmoregulatory) were studied using the Box-Behnken Design methodology with 4-factors and 3-levels. The best conditions were calculated by statistical regression analysis and surface plots. The optimal production parameters for maximum glycerol production were at 33.34ºC, 6.9pH, 22.454 % sugar concentration and 6.307 % initial glycerol as osmoregulator. The concentration of glycerol and residual sugars under these optimal conditions were 129.65 and 7.404 gl-1, respectively. Results recorded during this study appeared that the osmotolerant W. anomalus AUMC 11687 is promising yeast with great potentials for application in glycerol production on large scale for industrial purposes.