Induction of Ascites in Mice
- 1 October 2021
- journal article
- Published by Cold Spring Harbor Laboratory in Cold Spring Harbor Protocols
- Vol. 2021 (10)
- https://doi.org/10.1101/pdb.prot100289
Abstract
Smaller animals such as rats, mice, hamsters, and guinea pigs are usually poor choices for polyclonal antibody production because only small volumes of serum can be obtained. This problem can be reduced by inducing the formation of ascites in mice, which can provide up to 10 mL of ascites fluid from a single animal. Antibody titers in ascites fluids are almost as high as serum titers.Keywords
This publication has 2 references indexed in Scilit:
- Inducing, Collecting, and Storing AscitesCold Spring Harbor Protocols, 2021
- A modified method to induce immune polyclonal ascites fluid in BALB/c mice using Sp2/0-Ag14 cellsJournal of Immunological Methods, 1986