A case–control study on the association of intestinal flora with ulcerative colitis
Open Access
- 15 July 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in AMB Express
- Vol. 11 (1), 1-12
- https://doi.org/10.1186/s13568-021-01267-9
Abstract
The association between intestinal flora and ulcerative colitis (UC) was studied in order to provide a basis and method for clinical treatment. Fresh fecal samples were collected from 30 active UC patients and 10 healthy controls. The intestinal flora DNA from each sample was extracted and 16S rRNA gene sequencing was carried out using HiSeq platform to identify the intestinal flora in fecal samples. The richness and diversity of intestinal flora in UC patients were significantly lower than those in healthy control group (P < 0.05). Significant differences were observed between the intestinal flora-species of UC patients and healthy controls. Synergistetes (P < 0.01) and Firmicutes (P < 0.05), along with probiotics Veillonella (P < 0.01), Ruminococcus and Coprococcus (P < 0.05) in the UC patients were lower than that in the healthy controls significantly. Furthermore, compared with the control group, Tenericutes (P < 0.01) and intestinal pathogenic bacteria, including Bacteroides (P < 0.01), Escherichia and Sutterella (P < 0.05) were significantly increased. The incidence of UC is significantly associated with the changes in intestinal flora. Changes in intestinal flora may lead to a decrease in the diversity of intestinal flora or to the enrichment of a particular intestinal flora.Keywords
Funding Information
- the General Program of Natural Science Foundation of Heilongjiang Province (No. D200826)
- the General Projects of Scientific Research and Innovation Fund of the First Affiliated Hospital of Harbin Medical University (No. 2017Y006)
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