The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens
Open Access
- 18 April 2016
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Genomics
- Vol. 17 (1), 1-18
- https://doi.org/10.1186/s12864-016-2612-7
Abstract
Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially reduce its subsequent entry into the food chain. Campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. In the present study, two chicken populations were used to investigate the genetic architecture of avian resistance to colonisation: (i) a back-cross of two White Leghorn derived inbred lines [(61 x N) x N] known to differ in resistance to Campylobacter colonisation and (ii) a 9th generation advanced intercross (61 x N) line. The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait. A back-cross experiment using 1,243 fully informative single nucleotide polymorphism (SNP) markers revealed quantitative trait loci (QTL) on chromosomes 7, 11 and 14. In the advanced intercross line study, the location of the QTL on chromosome 14 was confirmed and refined and two new QTLs were identified located on chromosomes 4 and 16. Pathway and re-sequencing data analysis of the genes located in the QTL candidate regions identified potential pathways, networks and candidate resistance genes. Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results. Campylobacter resistance in chickens is a complex trait, possibly involving the Major Histocompatibility Complex, innate and adaptive immune responses, cadherins and other factors. Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.Funding Information
- Biotechnology and Biological Sciences Research Council (BB/J006815/1)
- Department for the Environment, Food and Rural Affairs (LINK grant no. LK0665)
- Sixth Framework Programme (SABRE - FOOD-CT-2006-01625)
This publication has 80 references indexed in Scilit:
- Development of a high density 600K SNP genotyping array for chickenBMC Genomics, 2013
- Distinct Roles of Secreted HtrA Proteases from Gram-negative Pathogens in Cleaving the Junctional Protein and Tumor Suppressor E-cadherin*Online Journal of Public Health Informatics, 2012
- Systems Analysis of Immune Responses in Marek's Disease Virus-Infected Chickens Identifies a Gene Involved in Susceptibility and Highlights a Possible Novel Pathogenicity MechanismJournal of Virology, 2011
- CpG islands and the regulation of transcriptionGenes & Development, 2011
- Fine mapping and replication of QTL in outbred chicken advanced intercross linesGenetics Selection Evolution, 2011
- Reactive arthritis following culture-confirmed infections with bacterial enteric pathogens in Minnesota and Oregon: a population-based studyAnnals Of The Rheumatic Diseases, 2008
- Gene expression profiling in chicken heterophils with Salmonella enteritidis stimulation using a chicken 44 K Agilent microarrayBMC Genomics, 2008
- Campylobacter-Induced Interleukin-8 Secretion in Polarized Human Intestinal Epithelial Cells RequiresCampylobacter-Secreted Cytolethal Distending Toxin- and Toll-Like Receptor-Mediated Activation of NF-κBInfection and Immunity, 2008
- PLINK: A Tool Set for Whole-Genome Association and Population-Based Linkage AnalysesAmerican Journal of Human Genetics, 2007
- Haploview: analysis and visualization of LD and haplotype mapsBioinformatics, 2004