PENGARUH PEMBERIAN CELLULOSE ACETATE DARI LAMUN LAUT (Enhalus acoroides) SEBAGAI SURFACE COATING PLAT RESIN AKRILIK TERHADAP JUMLAH KOLONI Candida albicans
DENTA , Volume 12; doi:10.30649/denta.v12i2.176
Abstract: Background : The denture base made of acrylic resin has a porosity and water absorbtion characteristic to cause the deposition of food waste, that problem was known to cause a growth of Candida albicans microorganisms. This problem can be solved by providing a coating (Surface coating) made of natural polymer (cellulose acrtate) of sea sea grass (Enhalus acoroides) mixed with hydrocoloid material so as to provide protection properties. Such protective properties can inhibit the bonding between the residual monomer results of acrylic resins and microorganisms. Objective : To analyze and compare the number of Candida albicans colonies on the acrylic resin plate that has surface coating and no surface coating. Method : Using post test only group design, using 12 samples divided into 2 groups. Group 1 is the control group (Heat cured acrylic plate contaminated with Candida albicans). Group 2 : Treatment group (Heat cured acrylic plate with surface coating made from sea grass Enhalus acoroides then contaminated with Cndida albicans). Calculating the number of colonies from Candida albicans was done by calculating the turbidity of SDB media (Saboraud Dextrose Broth) using UV-VIS Specrofotometer with 560 λ wave. Data analysis using an Independent T-Test. Results : Acrylic resin plates with surface coating showed significant differences in the number of Candida albicans colonies. The average results in the control group obtained an average number of colonies of 1,58 CFU/ml, while in the treatment group as much as 1,42 CFU/ml. Conclusion : The results show that surface coating can decrease the amount of Candida albicans colonies because it can close the microporosity, prevent a water absorption, and prevent the formation of the pellicle.
Keywords: Candida albicans / surface coating / microorganisms / Acrylic Resin / Resin Plate
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