The influence of covalent immobilization conditions on antibody accessibility on nanoparticles
Open Access
- 17 October 2017
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 142 (22), 4247-4256
- https://doi.org/10.1039/c7an01424d
Abstract
Antibody accessibility on the nanoparticle surface depends on the surface density rather than on the coupling conditions.Keywords
Funding Information
- FP7 People: Marie-Curie Actions (264737)
This publication has 29 references indexed in Scilit:
- Nano-visualization of oriented-immobilized IgGs on immunosensors by high-speed atomic force microscopyScientific Reports, 2012
- The Controlled Display of Biomolecules on Nanoparticles: A Challenge Suited to Bioorthogonal ChemistryBioconjugate Chemistry, 2011
- Taking Advantage of Unspecific Interactions to Produce Highly Active Magnetic Nanoparticle−Antibody ConjugatesACS Nano, 2011
- In-Depth Investigation of Protein Adsorption on Gold Surfaces: Correlating the Structure and Density to the Efficiency of the Sensing LayerThe Journal of Physical Chemistry B, 2008
- National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines: Clinical Characteristics and Utilization of Biochemical Markers in Acute Coronary SyndromesClinical Chemistry, 2007
- Orientation of a Monoclonal Antibody Adsorbed at the Solid/Solution Interface: A Combined Study Using Atomic Force Microscopy and Neutron ReflectivityLangmuir, 2006
- Effect of Surface Packing Density of Interfacially Adsorbed Monoclonal Antibody on the Binding of Hormonal Antigen Human Chorionic GonadotrophinThe Journal of Physical Chemistry B, 2006
- Stabilization of Yeast Cytochrome c Covalently Immobilized on Fused Silica SurfacesJournal of the American Chemical Society, 2004
- Epoxy-Amino Groups: A New Tool for Improved Immobilization of Proteins by the Epoxy MethodBiomacromolecules, 2003
- A Method for Studying Protein Orientation with Atomic Force Microscopy Using Relative Protein VolumesThe Journal of Physical Chemistry B, 2001