Adaptive Mechanisms of Somatostatin-Positive Interneurons after Traumatic Brain Injury through a Switch of α Subunits in L-Type Voltage-Gated Calcium Channels
- 19 August 2021
- journal article
- research article
- Published by Oxford University Press (OUP) in Cerebral Cortex
- Vol. 32 (5), 1093-1109
- https://doi.org/10.1093/cercor/bhab268
Abstract
Unilateral traumatic brain injury (TBI) causes cortical dysfunctions spreading to the primarily undamaged hemisphere. This phenomenon, called transhemispheric diaschisis, is mediated by an imbalance of glutamatergic versus GABAergic neurotransmission. This study investigated the role of GABAergic, somatostatin-positive (SST) interneurons in the contralateral hemisphere 72 h after unilateral TBI. The brain injury was induced to the primary motor/somatosensory cortex of glutamate decarboxylase 67–green fluorescent protein (GAD67-GFP) knock-in mice at postnatal days 19–21 under anesthesia in vivo. Single GFP+ interneurons of the undamaged, contralateral cortex were isolated by fluorescence-activated cell sorting and analyzed by mass spectrometry. TBI caused a switch of 2 α subunits of pore-forming L-type voltage-gated calcium channels (VGCC) in GABAergic interneurons, an increased expression of CaV1.3, and simultaneous ablation of CaV1.2. This switch was associated with 1) increased excitability of single SST interneurons in patch-clamp recordings and (2) a recovery from early network hyperactivity in the contralateral hemisphere in microelectrode array recordings of acute slices. The electrophysiological changes were sensitive to pharmacological blockade of CaV1.3 (isradipine, 100 nM). These data identify a switch of 2 α subunits of VGCCs in SST interneurons early after TBI as a mechanism to counterbalance post-traumatic hyperexcitability.Funding Information
- German Research Foundation (CRC 1080 C02)
This publication has 63 references indexed in Scilit:
- The L-type channel antagonist isradipine is neuroprotective in a mouse model of Parkinson's diseaseNeurobiology of Disease, 2011
- Robust L‐type calcium current expression following heterozygous knockout of the Cav1.2 gene in adult mouse heartJournal Of Physiology-London, 2011
- Functional Consequences of the Disturbances in the GABA-Mediated Inhibition Induced by Injuriesin the Cerebral CortexNeural Plasticity, 2011
- Three groups of interneurons account for nearly 100% of neocortical GABAergic neuronsDevelopmental Neurobiology, 2010
- Glutamate Receptor Ion Channels: Structure, Regulation, and FunctionPharmacological Reviews, 2010
- A robust and high-throughput Cre reporting and characterization system for the whole mouse brainNature Neuroscience, 2009
- Impaired long-term potentiation and enhanced neuronal excitability in the amygdala of CaV1.3 knockout miceNeurobiology of Learning and Memory, 2009
- Chronic Traumatic Encephalopathy in Athletes: Progressive Tauopathy After Repetitive Head InjuryJournal of Neuropathology and Experimental Neurology, 2009
- Animal Models of Post-Traumatic EpilepsyJournal of Neurotrauma, 2006
- Experimental Studies with Isradipine in StrokeDrugs, 1990