Evaluation of Silicon Phthalocyanine 4 Photodynamic Therapy against Human Cervical Cancer Cells in Vitro and in Mice
Open Access
- 1 January 2016
- journal article
- research article
- Published by Scientific Research Publishing, Inc. in Advances in Biological Chemistry
- Vol. 06 (06), 193-215
- https://doi.org/10.4236/abc.2016.66017
Abstract
Background: Cervical cancer is the second most common cancer in women worldwide [1]. Photodynamic therapy has been used for cervical intraepithelial neoplasia with good responses, but few studies have used newer phototherapeutics. We evaluated the effectiveness of photodynamic therapy using Pc 4 in vitro and in vivo against human cervical cancer cells. Methods: CaSki and ME-180 cancer cells were grown as monolayers and spheroids. Cell growth and cytotoxicity were measured using a methylthiazol tetrazolium assay. Pc 4 cellular uptake and intracellular distribution were determined. For in vitro Pc 4 photodynamic therapy, cells were irradiated at 667 nm at a fluence of 2.5 J/cm2 at 48 h. SCID mice were implanted with CaSki and ME-180 cells both subcutaneously and intracervically. Forty-eight hours after Pc 4 photodynamic therapy was administered at 75 and 150 J/cm2. Results: The IC50s for Pc 4 and Pc 4 photodynamic therapy for CaSki and ME-180 cells as monolayers were, 7.6 μM and 0.016 μM and >10 μM and 0.026 μM; as spheroids, IC50s of Pc 4 photodynamic therapy were, 0.26 μM and 0.01 μM. Pc 4 was taken up within cells and widely distributed in tumors and tissues. Intracervical photodynamic therapy resulted in tumor death, however mice died due to gastrointestinal toxicity. Photodynamic therapy resulted in subcutaneous tumor death and growth delay. Conclusions: Pc 4 photodynamic therapy caused death within cervical cancer cells and xenografts, supporting development of Pc 4 photodynamic therapy for treatment of cervical cancer. Support: P30-CA47904, CTSI BaCCoR Pilot Program.Keywords
This publication has 23 references indexed in Scilit:
- A Cell-Targeted Photodynamic Nanomedicine Strategy for Head and Neck CancersMolecular Pharmaceutics, 2013
- Gastrointestinal transit measurements in mice with 99mTc-DTPA-labeled activated charcoal using NanoSPECT-CTEJNMMI Research, 2013
- Synergistic Anti-Tumor Effects of Combination of Photodynamic Therapy and Arsenic Compound in Cervical Cancer Cells: In Vivo and In Vitro StudiesPLOS ONE, 2012
- Cell Death Pathways in Photodynamic Therapy of CancerCancers, 2011
- Successful cutaneous delivery of the photosensitizer silicon phthalocyanine 4 for photodynamic therapyClinical and Experimental Dermatology, 2011
- Silicon phthalocyanine (pc 4) photodynamic therapy is a safe modality for cutaneous neoplasms: results of a phase 1 clinical trialLasers in Surgery and Medicine, 2010
- The Relationship of Phthalocyanine 4 (Pc 4) Concentrations Measured Noninvasively to Outcome of Pc 4 Photodynamic Therapy in MicePhotochemistry and Photobiology, 2009
- Clinical Pharmacokinetics of the PDT Photosensitizers Porfimer Sodium (Photofrin), 2‐[1‐Hexyloxyethyl]‐2‐Devinyl Pyropheophorbide‐a (Photochlor) and 5‐ALA‐Induced Protoporphyrin IXLasers in Surgery and Medicine, 2006
- Epidemiologic Classification of Human Papillomavirus Types Associated with Cervical CancerThe New England Journal of Medicine, 2003
- SQUAMOUS CELL CARCINOMA OF THE CERVIX WITH EXTENSIVE SUPERFICIAL SPREADING TO ALMOST WHOLE GENITAL TRACT AND ASSOCIATED WITH ENDOMETRIAL STROMAL SARCOMAActa Pathologica Japonica, 1988