The effect of conservation in various cryoprotectants on the properties and morphological structure of the umbilical cord arteries
Open Access
- 17 August 2022
- journal article
- Published by QUASAR, LLC in Research and Practical Medicine Journal
- Vol. 9 (3), 106-116
- https://doi.org/10.17709/2410-1893-2022-9-3-8
Abstract
Purpose of the study. The aim of this work was to study the effect of conservation in various cryoprotectants on the biomechanical and morphological properties of the umbilical cord (UCA) arteries.Materials and methods. The material for this work was 12 umbilical arteries, which were divided into 4 study groups: (1) native UCA – control without freezing; (2) APC frozen in 5 % dextran with the addition of dimethyl sulfoxide (DMSO) to 10 % and 7.5 % human serum albumin (HSA); (3) APC frozen in glycerin and (4) UCA frozen in propanediol. For all groups, morphological and biomechanical studies were carried out. The statistical data were processed using the GraphPad Prism 9.2.0 program. Analysis of variance was used to find significant differences.Results. Freezing with 5 % dextran, 10 % DMSO and 7.5 % human albumin did not lead to significant changes in the morphology of vascular tissue compared to control. The conserved vascular tissue retains a moderate level of type I collagen, regardless of the method of conservation. The presence of statistically significant differences between the control and frozen UCAs was shown for Young's modulus (p < 0.05; 6.9 [6.8; 7.0] for the native artery; 2.3 [2.0; 2.6] for the artery preserved with the use of dextran, DMSO and human albumin; 2.3 [1.4; 3.2] for the artery preserved in glycerin and 0.8 [0.6; 1.0] – in propanediol, respectively).Conclusion. 1) Freezing and storage of UCA at 5 % dextran, 10 % DMSO and 7.5 % HSA does not lead to a significant change in the morphology of vascular membranes and cell nuclei; 2) The preservation of type I collagen according to the results of IHC is qualitatively reduced from a conditional indicator of +2 to +1 for frozen agroindustrial complexes compared with control ones; 3) Conservation leads to a significant decrease in the modulus of elasticity of the walls of the agroindustrial complex compared with control vessels (p < 0.05).Keywords
This publication has 14 references indexed in Scilit:
- Considerations in the Development of Small-Diameter Vascular Graft as an Alternative for Bypass and Reconstructive Surgeries: A ReviewCardiovascular Engineering and Technology, 2020
- Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech RepublicAdvances in Clinical and Experimental Medicine, 2019
- Allograft Tissue Safety and TechnologyPublished by Elsevier BV ,2018
- Umbilical cord tissue cryopreservation: a short reviewStem Cell Research & Therapy, 2018
- Current insights into skin banking: storage, preservation and clinical importance of skin allograftsJournal of Biorepository Science for Applied Medicine, 2017
- Banking of cryopreserved iliac artery and vein homografts: clinical uses in transplantationCell and Tissue Banking, 2014
- Optimizing isolation culture and freezing methods to preserve Wharton's jelly's mesenchymal stem cell (MSC) properties: an MSC banking protocol validation for the Hellenic Cord Blood BankTransfusion, 2014
- Potential for polyhydroxyalkanoates and policaprolactone copolymer use as tissue-engineered scaffolds in cardiovascular surgeryBulletin of Siberian Medicine, 2012
- Dacron® vs. PTFE as bypass materials in peripheral vascular surgery – systematic review and meta-analysisBMC Surgery, 2008
- Long-term Results of Endovascular AAA Repair Using a Homemade Aortomonoiliac PTFE DeviceJournal of Endovascular Therapy, 2002