A novel label-free fluorescence assay for dipeptidyl peptidase 4 activity detection based on supramolecular self-assembly
- 7 January 2020
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 56 (11), 1629-1632
- https://doi.org/10.1039/c9cc09053c
Abstract
This article presents a new label-free fluorescence assay based on supramolecular self-assembly of cucurbit[7]uril and specific peptide Gly-Pro-Phe-Gly for monitoring DPP4 activity in clinical samples. It also displays a good potential application in high-throughput screening of DPP4 inhibitors.Keywords
Funding Information
- National Natural Science Foundation of China (81774151, 81822047)
- Fundamental Research Funds for the Central Universities (2019XZZX004-16)
- Health and Family Planning Commission of Sichuan Province (1701-02, 201840244)
- Zhejiang University
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