Cell Culture System for the Study of the Hepatitis E Virus

Abstract

戊型肝炎病毒(HEV)是人类戊型肝炎的致病因子，也是全世界肠道传播的病毒性肝炎的主要原因。病毒学研究需要强大的细胞培养系统。不幸的是，与其他肝炎病毒一样，HEV难以在常规细胞中繁殖。虽然已经使用各种HEV毒株测试了许多不同的细胞培养系统，但是病毒复制通常进展非常缓慢，并且具有低病毒粒子计数的感染导致HEV复制无效。然而，最近发现在不同细胞系中传代原发患者分离株的进展改善了体外HEV传播。本综述描述了几个典型毒株的细胞培养情况。 Hepatitis E virus (HEV) is the causative agent of human hepatitis E and the main cause of viral hepatitis transmitted in the intestines worldwide. Virology research requires a powerful cell culture system. Unfortunately, like other hepatitis viruses, HEV is difficult to reproduce in conventional cells. Many different cell culture systems have been tested using various HEV strains, but virus replication generally progresses very slowly, and infections with low virion counts render HEV replication ineffective. However, recent advances in the passage of primary patient isolates in different cell lines have improved in vitro HEV transmission. This review describes the cell culture of several typical strains.