Effects of Salicylic Acid on the Growth of Rice Seedlings and Its Regulation Mechanism

Abstract

为了探究不同浓度的水杨酸(salicylic acid, SA)对水稻幼苗生长的影响及其调控机制，本研究采用DR5::GUS转基因水稻(Oryza sativa L.，中花11)为研究材料，观察不同浓度SA对水稻生长的影响，同时检测其中生长素的时空分布变化，以及检测生长素合成运输基因表达水平的变化。研究结果显示，低浓度SA (10 µM)促进水稻幼苗生长，高浓度SA (1 mM)抑制水稻幼苗的生长。低浓度SA和高浓度SA的处理影响了水稻幼苗根中生长素的时空分布状态，并且影响了生长素合成和运输基因的表达水平。因此，不同浓度的SA可能通过调控了生长素的时空分布参与调控了水稻幼苗的生长调节过程。In order to investigate the different concentration of salicylic acid (SA) on rice seedling growth and its regulatory mechanism, this study used DR5::GUS transgenic rice (Oryza sativa L., Zhonghua 11) as material to observe the effect of different concentrations of SA on rice growth, on auxin spatial and temporal distribution, and on expression level of auxin synthesis and transport genes. The results show that low concentration of SA (10 µM) promotes the growth of rice seedlings, and high concentration of SA (1 mM) inhibits the growth of rice seedlings. The treatment of low concentration of SA and high concentration of SA affects the spatial and temporal distribution of auxin in the roots of rice seedlings, and affects the expression level of the auxin synthesis and transport genes. Therefore, different concentrations of SA may be involved in the regulation of rice seedling growth by regulating the spatial and temporal distribution of auxin.