Probiotics can be considered as biological agents that modify the intestinal microbiota as well as show several health benefits. The purpose of this study was to explore the effects of probiotics in neonatal STZ-induced type-2 diabetic rats. Following the biochemical analysis of probiotic bacteria, yogurt was prepared using cow milk and used to feed the experimental rats. Probiotic yogurt was supplemented to three rat groups, each with six rats, and the doses were 2 g in PYT1, 4 g in PYT2 and 6 g in PYT3 for 21 days. Fasting serum glucose, serum insulin, lipid profiles and liver glycogen levels were measured to investigate the probiotic effects on type 2 diabetic rats. It was observed that fasting serum glucose levels were significantly lower in case of PYT3 group (pppp<0.05) increased level of HDL was detected in PYT1 and PYT2 rat groups. However, there were no significant differences regarding triglyceride as well as total cholesterol levels among three probiotic yogurt supplemented rat groups. Hepatic glycogen content was 34.5%, 30.9% and 39.1% among the probiotic yogurt feed groups viz. PYT1, PYT2 and PYT3, respectively. Findings of this research suggest that application of probiotic yogurt can help to manage type-2 diabetes. Further study on clinical trial would be worthy to investigate for probiotic-based product improvement for treatment of type-2 diabetic patients.