An integrated strategy for designing and fabricating triple-layer vascular graft with oriented microgrooves to promote endothelialization
- 12 March 2021
- journal article
- research article
- Published by SAGE Publications in Journal of Biomaterials Applications
- Vol. 36 (2), 297-310
- https://doi.org/10.1177/08853282211001006
Abstract
Compared with native blood vessels and existing vascular grafts, design and manufacture of vascular grafts with a three-dimensional topological structure is a key to induce cells and tissue growth, which remains an essential issue in both tissue engineering and regenerative medicine. This study sought to develop a novel triple-layer vascular graft (TLVG) with oriented microgrooves to investigate the mechanical property and endothelialization. The TLVGs were composed of electrospun Poly-ε-caprolactone (PCL)/thermoplastic polyurethane (TPU) as the inner layer, albumen/sodium alginate (SA) hydrogel as the middle layer, and electrospun PCL/TPU as the outer layer. In detail, a cylindrical sacrificial template was designed and printed using polyvinyl alcohol (PVA), served as the electrospinning receiving platform to form the oriented microgrooves in the inner layer of TLVGs. The highly elastic albumen/SA hydrogel and PCL/TPU nanofibers were able to simulate the elastin in blood vessels. In addition, the introduction of the albumen/SA hydrogel layer not only solves the leakage problem of a porous vascular graft but also improves the wettability of the scaffolds. The physicochemical properties and biological characteristics of TLVGs were evaluated by tensile testing, Surface wettability test, Fourier transform-infrared spectroscopy (FTIR) measurement, Live-Dead cell staining assay, and CCK-8 assay. Especially, the oriented microgrooves on the inner surface of the TLVGs can promote human umbilical vein endothelial cells (HUVECs) directed growth and migration in favor of endothelialization. All results showed that the fabricated TLVGs with excellent physicochemical properties and biocompatibility has great potential in clinic application.Keywords
Funding Information
- National Natural Science Foundation of China (51775324)
This publication has 38 references indexed in Scilit:
- The Vascular Endothelium and Human DiseasesInternational Journal of Biological Sciences, 2013
- Electrospinning collagen/chitosan/poly(L‐lactic acid‐co‐ϵ‐caprolactone) to form a vascular graft: Mechanical and biological characterizationJournal of Biomedical Materials Research Part A, 2012
- Cardiovascular Disease in the Developing WorldJournal of Invasive Cardiology, 2012
- Fabrication of channeled scaffolds with ordered array of micro-pores through microsphere leaching and indirect Rapid Prototyping techniqueBiomedical Microdevices, 2012
- Endothelialization and patency of RGD-functionalized vascular grafts in a rabbit carotid artery modelBiomaterials, 2012
- Role of prosthetic conduits in coronary artery bypass graftingEuropean Journal of Cardio-Thoracic Surgery, 2011
- Image Analysis of Endothelial Microstructure and Endothelial Cell Dimensions of Human Arteries – A Preliminary StudyAdvanced Engineering Materials, 2010
- Mechanical properties of completely autologous human tissue engineered blood vessels compared to human saphenous vein and mammary arteryBiomaterials, 2009
- Alginate gels: I. Characterization of textural attributesJournal of Food Engineering, 2008
- Modulation of anisotropy in electrospun tissue-engineering scaffolds: Analysis of fiber alignment by the fast Fourier transformBiomaterials, 2006