Enrichment of Hydrogen-Oxidizing Bacteria from High-Temperature and High-Salinity Environments
- 29 January 2021
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 87 (4)
- https://doi.org/10.1128/aem.02439-20
Abstract
There is an urgent need for sustainable protein supply routes with low environmental footprint. Recently, the use of hydrogen oxidizing bacteria (HOB) as a platform for high quality microbial protein (MP) production has regained interest. This study aims to investigate the added value of using conditions such as salt and temperature to steer HOB communities to lower diversities, while maintaining a high protein content and a high quality amino acid profile. Pressure drop and hydrogen consumption were measured for 56 days to evaluate autotrophy of a total of six communities in serum flasks. Of the six communities, four were enriched under saline (0.0, 0.25, 0.5 and 1.0 mol NaCl l−1) and two under thermophilic conditions (65°C). Five communities enriched for HOB were subsequently cultivated in continuously stirred reactors under the same conditions to evaluate their potential as microbial protein producers. The protein percentages ranged from 41 to 80%. The highest protein content was obtained for the thermophilic enrichments. Amino acid profiles were comparable to protein sources commonly used for feed purposes. Members of the genus Achromobacter were found to dominate the saline enrichments while members of the genus Hydrogenibacillus were found to dominate the thermophilic enrichments. Here we show that enriching for HOB while steering the community toward low diversity and maintaining a high quality protein content can be successfully achieved, both in saline and thermophilic conditions. IMPORTANCE Alternative feed and food supply chains are required to decrease water and land use. HOB offer a promising substitute for traditional agricultural practice to produce microbial protein (MP) from residual materials and renewable energy. To safeguard product stability, the composition of the HOB community should be controlled. Defining strategies to maintain the stability of the communities is therefore key for optimization purposes. In this study, we use salt and temperature as independent conditions to stabilize the composition of the HOB communities. Based on the results presented, we conclude that HOB communities can be steered to have low diversity using the presented conditions while producing a desirable protein content with a valuable amino acid profile.Keywords
Funding Information
- European Union's Horizon 2020 research and innovation program under the arie Sklododowska-Curie (665874)
- Geconcentreerde Onderzoekactie, Ghent University (BOF15/GOA/006)
This publication has 54 references indexed in Scilit:
- phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census DataPLOS ONE, 2013
- The SILVA ribosomal RNA gene database project: improved data processing and web-based toolsNucleic Acids Research, 2012
- Campylobacter spp. as a Foodborne Pathogen: A ReviewFrontiers in Microbiology, 2011
- phangorn: phylogenetic analysis in RBioinformatics, 2010
- Halophiles 2010: Life in Saline EnvironmentsApplied and Environmental Microbiology, 2010
- Resistance, resilience, and redundancy in microbial communitiesProceedings of the National Academy of Sciences of the United States of America, 2008
- Potential and utilization of thermophiles and thermostable enzymes in biorefiningMicrobial Cell Factories, 2007
- Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16Nature Biotechnology, 2006
- Nutraceuticals and functional foods regulations in the United States and around the worldToxicology, 2006
- Bacillus schlegelii, a New Species of Thermophilic, Facultatively Chemolithoautotrophic Bacterium Oxidizing Molecular HydrogenJournal of General Microbiology, 1979