A carotenoid-deficient mutant of the plant-associated microbe Pantoea sp. YR343 displays an altered membrane proteome
Open Access
- 11 September 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 10 (1), 1-21
- https://doi.org/10.1038/s41598-020-71672-w
Abstract
Membrane organization plays an important role in signaling, transport, and defense. In eukaryotes, the stability, organization, and function of membrane proteins are influenced by certain lipids and sterols, such as cholesterol. Bacteria lack cholesterol, but carotenoids and hopanoids are predicted to play a similar role in modulating membrane properties. We have previously shown that the loss of carotenoids in the plant-associated bacteria Pantoea sp. YR343 results in changes to membrane biophysical properties and leads to physiological changes, including increased sensitivity to reactive oxygen species, reduced indole-3-acetic acid secretion, reduced biofilm and pellicle formation, and reduced plant colonization. Here, using whole cell and membrane proteomics, we show that the deletion of carotenoid production in Pantoea sp. YR343 results in altered membrane protein distribution and abundance. Moreover, we observe significant differences in the protein composition of detergent-resistant membrane fractions from wildtype and mutant cells, consistent with the prediction that carotenoids play a role in organizing membrane microdomains. These data provide new insights into the function of carotenoids in bacterial membrane organization and identify cellular functions that are affected by the loss of carotenoids.Keywords
Funding Information
- U.S. Department of Energy
This publication has 80 references indexed in Scilit:
- Making a beta-barrel: assembly of outer membrane proteins in Gram-negative bacteriaCurrent Opinion in Microbiology, 2012
- At the membrane frontier: A prospectus on the remarkable evolutionary conservation of polyprenols and polyprenyl-phosphatesArchives of Biochemistry and Biophysics, 2012
- Faster SEQUEST Searching for Peptide Identification from Tandem Mass SpectraJournal of Proteome Research, 2011
- The Crystal Structure of BamB Suggests Interactions with BamA and Its Role within the BAM ComplexJournal of Molecular Biology, 2011
- Flipping Lipids: Why an’ What’s the Reason for?ACS Chemical Biology, 2009
- ClueGO: a Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networksBioinformatics, 2009
- Sterol carrier protein-2: New roles in regulating lipid rafts and signalingBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2007
- Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics researchBioinformatics, 2005
- Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction NetworksGenome Research, 2003
- Genome‐wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organismsProtein Science, 1998