Efficient Neural Differentiation of Mouse Embryonic Stem Cells by Mastic Gum
- 1 February 2023
- journal article
- research article
- Published by Mary Ann Liebert Inc in Biopreservation and Biobanking
- Vol. 21 (1), 38-45
- https://doi.org/10.1089/bio.2021.0130
Abstract
Purpose: Promoting neurogenesis is a promising strategy to treat neurodegenerative disorders. In the present study, we aimed to evaluate the effect of mastic gum resin from the Pistacia lentiscus var. Chia (Anacardiaceae family) in proliferation capacity and differentiation of embryonic mesenchymal stem cells into a neural lineage. Methods: For this purpose, mastic gum was applied as a neural inducer for stem cell differentiation into the neuronal lineage. Following treatment of embryonic stem cells (ESCs) with mastic gum, verification differentiation of the ESCs into the neuronal lineage, gene expression analysis, and immunocytochemistry staining approach were performed. Results: Gene expression analysis demonstrated that mastic gum increased the expression level of neuron markers in the ESCs-derived neuron-like cells. Moreover, our immunocytochemistry staining results of two important neural stem cell markers, including Nestin and microtubule-associated protein-2 (Map2) expression confirmed that mastic gum has the potential to promote neuronal differentiation in ESCs. Conclusion: In summary, the use of mastic gum to stimulate the differentiation of ESCs into a neural lineage can be considered as a good candidate in stem cell therapy.Keywords
This publication has 43 references indexed in Scilit:
- Multiple sclerosis and mitochondrial gene variations: A reviewJournal of the Neurological Sciences, 2013
- The synergistic effect of beta-boswellic acid and Nurr1 overexpression on dopaminergic programming of antioxidant glutathione peroxidase-1-expressing murine embryonic stem cellsNeuroscience, 2012
- Induction of murine embryonic stem cell differentiation by medicinal plant extractsExperimental Cell Research, 2011
- Mechanisms of Vascular Aging: New PerspectivesThe Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 2010
- Human Embryonic Stem Cell Differentiation Toward Regional Specific Neural PrecursorsThe International Journal of Cell Cloning, 2009
- Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cellsNature Biotechnology, 2007
- Dynamic changes in mitochondrial biogenesis and antioxidant enzymes during the spontaneous differentiation of human embryonic stem cellsBiochemical and Biophysical Research Communications, 2006
- Chemical Composition and Antibacterial Activity of the Essential Oil and the Gum ofPistacia lentiscusVar. chiaJournal of Agricultural and Food Chemistry, 2005
- Conversion of embryonic stem cells into neuroectodermal precursors in adherent monocultureNature Biotechnology, 2003
- In vitro differentiation of transplantable neural precursors from human embryonic stem cellsNature Biotechnology, 2001