Opening Study on the Development of a New Biosensor for Metal Toxicity Based on Pseudomonas fluorescens Pyoverdine
Open Access
- 10 December 2013
- journal article
- research article
- Published by MDPI AG in Biosensors
- Vol. 3 (4), 385-399
- https://doi.org/10.3390/bios3040385
Abstract
To date, different kinds of biosensing elements have been used effectively for environmental monitoring. Microbial cells seem to be well-suited for this task: they are cheap, adaptable to variable field conditions and give a measurable response to a broad number of chemicals. Among different pollutants, heavy metals are still a major problem for the environment. A reasonable starting point for the selection of a biorecognition element to develop a biosensor for metals could be that of a microorganism that exhibits good mechanisms to cope with metals. Pseudomonads are characterized by the secretion of siderophores (e.g., pyoverdine), low-molecular weight compounds that chelate Fe3+ during iron starvation. Pyoverdine is easily detected by colorimetric assay, and it is suitable for simple online measurements. In this work, in order to evaluate pyoverdine as a biorecognition element for metal detection, the influence of metal ions (Fe3+, Cu2+, Zn2+), but also of temperature, pH and nutrients, on microbial growth and pyoverdine regulation has been studied in P. fluorescens. Each of these variables has been shown to influence the synthesis of siderophore: for instance, the lower the temperature, the higher the production of pyoverdine. Moreover, the concentration of pyoverdine produced in the presence of metals has been compared with the maximum allowable concentrations indicated in international regulations (e.g., 98/83/EC), and a correlation that could be useful to build a colorimetric biosensor has been observed.Keywords
This publication has 38 references indexed in Scilit:
- Epiphytic lichens as biomonitors of airborne heavy metal pollutionEnvironmental and Experimental Botany, 2013
- Pyoverdine biosynthesis and secretion in Pseudomonas aeruginosa: implications for metal homeostasisEnvironmental Microbiology, 2012
- Optimal pyoverdin-CPG composites for development of an optical biosensor to detect ironBiochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, 2012
- Iron specificity of a biosensor based on fluorescent pyoverdin immobilized in sol-gel glassJournal of Biological Engineering, 2011
- Siderophores mediate reduced and increased uptake of cadmium byStreptomyces tendaeF4 and sunflower (Helianthus annuus), respectivelyJournal of Applied Microbiology, 2009
- Microbial Iron Acquisition: Marine and Terrestrial SiderophoresChemical Reviews, 2009
- Siderotyping of fluorescent Pseudomonas: molecular mass determination by mass spectrometry as a powerful pyoverdine siderotyping methodBioMetals, 2007
- Geomycology: biogeochemical transformations of rocks, minerals, metals and radionuclides by fungi, bioweathering and bioremediationMycological Research, 2007
- Survival and Growth in the Presence of Elevated Copper: Transcriptional Profiling of Copper-Stressed Pseudomonas aeruginosaJournal of Bacteriology, 2006
- The Fluorescent Pigment of Pseudomonas fluorescens: Biosynthesis, Purification and Physicochemical PropertiesJournal of General Microbiology, 1978