Susceptibility to Acaricides and the Frequencies of Point Mutations in Etoxazole- and Pyridaben-Resistant Strains and Field Populations of the Two-Spotted Spider Mite, Tetranychus urticae (Acari: Tetranychidae)
Open Access
- 20 July 2021
- Vol. 12 (7), 660
- https://doi.org/10.3390/insects12070660
Abstract
The two-spotted spider mite Tetranychus urticae Koch is a major agricultural pest worldwide and is known to rapidly develop resistance to pesticides. In the present study, we explored a field strain that was collected in 2000 and 2003 and has been exhibiting resistance to etoxazole and pyridaben over the last 16 years. The resistance ratios of the etoxazole- and pyridaben-resistant strains (ER and PR) to etoxazole or pyridaben were more than 5,000,000- and 4109.6-fold higher than that of the susceptible strain, respectively. All field-collected populations showed resistance to etoxazole and pyridaben. The ER and PR strains showed cross-resistance to several acaricides. Both I1017F and H92R point mutations were detected in 7 out of 8 field groups. Spirodiclofen and spiromesifen resulted in more than 77.5% mortality in the 8 field groups. In addition, the genotype frequency of the I1017F point mutation was 100.0% in the ER strain, and that of the H92R point mutation was 97.0% in the PR strain. All of the field populations were found to have a high frequency of I1017F. These results suggest that the observation of resistance patterns will help in designing a sustainable IPM program for T. urticae.Keywords
This publication has 35 references indexed in Scilit:
- Status of pesticide resistance and associated mutations in the two-spotted spider mite, Tetranychus urticae, in ChinaPesticide Biochemistry and Physiology, 2018
- First detection of etoxazole resistance in Australian two‐spotted mite Tetranychus urticae Koch (Acarina: Tetranychidae) via bioassay and DNA methodsAustral Entomology, 2017
- Molecular diagnostics for detecting pyrethroid and abamectin resistance mutations in Tetranychus urticaePesticide Biochemistry and Physiology, 2017
- Monitoring Tetranychus urticae Koch (Acari: Tetranychidae) resistance to abamectin in vineyards in the Lower Middle São Francisco ValleyCrop Protection, 2015
- Global distribution and origin of target site insecticide resistance mutations in Tetranychus urticaeInsect Biochemistry and Molecular Biology, 2014
- Population bulk segregant mapping uncovers resistance mutations and the mode of action of a chitin synthesis inhibitor in arthropodsProceedings of the National Academy of Sciences of the United States of America, 2012
- Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari: A reviewInsect Biochemistry and Molecular Biology, 2010
- Acaricide mode of actionPest Management Science, 2004
- Monitoring of Acaricide Resistance in Two-Spotted Spider Mite (Tetranychus urticae) Populations from Rose Greenhouses in KoreaJournal of Asia-Pacific Entomology, 2003
- Synthesis and Activity of Novel Acaricidal/Insecticidal 2, 4-Diphenyl-1, 3-oxazolinesJournal of Pesticide Science, 2002