Involvement of integrin-linked kinase in capillary/tube-like network formation of human vascular endothelial cells
- 1 January 2005
- journal article
- Published by Springer Science and Business Media LLC in Biological Procedures Online
- Vol. 7 (1), 41-47
- https://doi.org/10.1251/bpo104
Abstract
Angiogenesis is a complex process involving an ECM and vascular endothelial cells (EC), and is regulated by various angiogenic factors including VEGF. The ability to form a capillary/tube-like network is a specialized function of EC. Therefore, in vitro angiogenesis was assessed by a capillary/tube-like network formation assay. There are three angiogenic parameters: capillary length, number of capillaries, and relative capillary area per field. We evaluated capillary length per field in the assay. VEGF promoted capillary/tube-like network formation of EC in a type I collagen gel matrix in vitro. Moreover, we demonstrated the involvement of ILK in a VEGF signaling pathway mediating capillary/tube-like network formation of EC using dominant-negative, kinase deficient ILK. This is a straightforward assay to monitor responses of human vascular endothelial cells.Keywords
This publication has 23 references indexed in Scilit:
- Integrin-Linked Kinase Regulates Endothelial Cell Survival and Vascular DevelopmentMolecular and Cellular Biology, 2004
- Integrin-linked kinase regulates vascular morphogenesis induced by vascular endothelial growth factorJournal of Cell Science, 2004
- Regulation of Protein Kinase B/Akt-Serine 473 Phosphorylation by Integrin-linked KinaseOnline Journal of Public Health Informatics, 2001
- Patterns and Emerging Mechanisms of the Angiogenic Switch during TumorigenesisCell, 1996
- Vascular endothelial growth factor and platelet-derived growth factor are potential angiogenic and metastatic factors in human breast cancerSurgery, 1996
- Regulation of cell adhesion and anchorage-dependent growth by a new β1-integrin-linked protein kinaseNature, 1996
- Integrins: Emerging Paradigms of Signal TransductionAnnual Review of Cell and Developmental Biology, 1995
- Identification of multiple active growth factors in basement membrane matrigel suggests caution in interpretation of cellular activity related to extracellular matrix componentsExperimental Cell Research, 1992
- Cell surface receptors for extracellular matrix componentsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1990
- A murine tumor producing a matrix of basement membrane.The Journal of Experimental Medicine, 1977