Ikan bandeng (Chanos chanos) di Waduk Sempor diintroduksi pada tahun 2014 melalui penerapan teknologi perikanan tangkap berbasis budidaya (Culture Based Fisheries, CBF). Penelitian ini bertujuan untuk mengevaluasi pertumbuhan bandeng melalui pengamatan parameter pertumbuhan dan biologi populasinya di Waduk Sempor, Jawa Tengah. Data yang dianalisis diperoleh dari hasil pencatatan enumerator periode September 2014 sampai– Desember 2015. Kajian dinamika populasi ikan bandeng yang meliputi laju pertumbuhan, laju kematian dan tingkat pemanfaatan mengacu pada persamaan Von Bertalanffy dan turunannya. Aspek biologi meliputi hubungan panjang berat mengacu pada model logistik, serta faktor kondisi, keseluruhan analisis dilakukan secara manual dengan program excel 2007. Hasil penelitian menunjukan bahwa parameter pertumbuhan panjang-asimtotik L∞ = 55,97 cm TL dan K= 0,38 per tahun. Laju kematian total (Z) = 1,17 per tahun, kematian alami (M) = 0,56 per tahun, kematian akibat penangkapan (F) = 0,61 per tahun serta laju eksploitasi (E) = 0,5. Pertumbuhan populasi dan laju pemanfaatan menunjukkan bahwa bandeng sudah dimanfaatkan secara optimal oleh masyarakat di sekitar Waduk Sempor. Milkfish (Chanos chanos) was introduced at 2014 in Sempor Reservoir through culture based fisheries (CBF) technology. This study aims to evaluate population growth of the milkfish in Sempor Reservoir. Data were collected from September 2015 to December 2016, and were analyzed using excel 2007 programe manually. Analysis of length-weight relationship is refers to logistic model, population dynamic like growth rate, mortality and mortality rate are refers to Von Bertalanffy and differentiate of it. Biology of population like length-weight relationship are refers to logistic model, and condition factor. The results showed that growth parameters were L∞ = 55.97 cm TL and K = 0.38 year-1. Total mortality (Z) = 1.17 year-1; M = 0.56 year-1; F = 0.61 year-1; and E=0.5. The growth rate and exploitation rate of Milkfish (Chanos chanos) in Sempor Reservoir has been utilized optimally.