Peeling back the layers of crassulacean acid metabolism: functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes
Open Access
- 21 April 2020
- journal article
- research article
- Published by Wiley in The Plant Journal
- Vol. 103 (2), 869-888
- https://doi.org/10.1111/tpj.14757
Abstract
Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that offers the potential to engineer improved water‐use efficiency (WUE) and drought resilience in C3 plants while sustaining productivity in the hotter and drier climates that are predicted for much of the world. CAM species show an inverted pattern of stomatal opening and closing across the diel cycle, which conserves water and provides a means of maintaining growth in hot, water‐limited environments. Recent genome sequencing of the constitutive model CAM species Kalanchoë fedtschenkoi provides a platform for elucidating the ensemble of proteins that link photosynthetic metabolism with stomatal movement, and that protect CAM plants from harsh environmental conditions. We describe a large‐scale proteomics analysis to characterize and compare proteins, as well as diel changes in their abundance in guard cell‐enriched epidermis and mesophyll cells from leaves of K. fedtschenkoi. Proteins implicated in processes that encompass respiration, the transport of water and CO2, stomatal regulation, and CAM biochemistry are highlighted and discussed. Diel rescheduling of guard cell starch turnover in K. fedtschenkoi compared with that observed in Arabidopsis is reported and tissue‐specific localization in the epidermis and mesophyll of isozymes implicated in starch and malate turnover are discussed in line with the contrasting roles for these metabolites within the CAM mesophyll and stomatal complex. These data reveal the proteins and the biological processes enriched in each layer and provide key information for studies aiming to adapt plants to hot and dry environments by modifying leaf physiology for improved plant sustainability.Keywords
Funding Information
- Biotechnology and Biological Sciences Research Council
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (31003A‐166539/1)
- Office of Science
- Oak Ridge National Laboratory
- Battelle
- National Science Foundation (31003A‐166539, 1)
This publication has 75 references indexed in Scilit:
- Overexpression of the aspartic protease ASPG1 gene confers drought avoidance in ArabidopsisJournal of Experimental Botany, 2012
- A CAM- and starch-deficient mutant of the facultative CAM species Mesembryanthemum crystallinum reconciles sink demands by repartitioning carbon during acclimation to salinityJournal of Experimental Botany, 2012
- Faster SEQUEST Searching for Peptide Identification from Tandem Mass SpectraJournal of Proteome Research, 2011
- Search and clustering orders of magnitude faster than BLASTBioinformatics, 2010
- Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca 2+ affinitiesProceedings of the National Academy of Sciences of the United States of America, 2010
- Carbonic anhydrases are upstream regulators of CO2-controlled stomatal movements in guard cellsNature, 2009
- ClueGO: a Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networksBioinformatics, 2009
- DAnTE: a statistical tool for quantitative analysis of -omics dataBioinformatics, 2008
- Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometryNature Methods, 2007
- Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics researchBioinformatics, 2005