Simultaneous Determination of Fenchone and Trans-Anethole in Essential Oils and Methanolic Extracts of Foeniculum vulgare Mill. Fruits Obtained from Different Geographical Regions Using GC-MS Approach
Open Access
- 23 May 2022
- journal article
- research article
- Published by MDPI AG in Separations
- Vol. 9 (5), 132
- https://doi.org/10.3390/separations9050132
Abstract
The gas chromatography–mass spectrometry (GC-MS) approach is established for the simultaneous determination of fenchone (FCO) and trans-anethole (TOH) in the essential oils and methanolic extracts of fennel (Foeniculum vulgare Mill.) fruits obtained from India (IND), Pakistan (PAK), and Saudi Arabia (SA). The simultaneous determination of FCO and TOH was performed via Agilent 190914S HP–5MS fused-silica capillary column (30 m × 250 µm ID, 0.25 µm film thickness). The proposed GC-MS approach was linear in the range of 0.10–50 µg/g for FCO and TOH. FCO’s detection (LOD) and quantification (LOQ) limits were calculated to be 0.04 and 0.12 µg/g, respectively. The LOD and LOQ values for TOH, on the other hand, were calculated to be 0.05 and 0.15 µg/g, respectively. In addition, the proposed GC-MS approach was accurate and precise for the simultaneous determination of FCO and TOH. The amount of FCO in essential oils of F. vulgare was computed as 0.021, 0.034, and 0.029 mg/g in the samples obtained from IND, PAK, and SA, respectively. The amount of TOH in the essential oils of F. vulgare was computed as 7.40, 14.8, and 10.2 mg/g in the samples obtained from IND, PAK, and SA, respectively. However, the amount of FCO in the methanolic extract of F. vulgare was estimated as 0.031, 0.021, and 0.057 mg/g in the samples obtained from IND, PAK, and SA, respectively. On the other hand, the amount of TOH in the methanolic extract of F. vulgare was estimated as 0.440, 0.498, and 1.74 mg/g in the samples obtained from IND, PAK, and SA, respectively. These findings suggested that the proposed GC-MS approach might be used to simultaneously determine the FCO and TOH in a variety of essential oils and plant extracts.Keywords
Funding Information
- King Saud University (RSP-2021/146)
This publication has 25 references indexed in Scilit:
- GC-MS Analysis and in Vitro Antimicrobial Susceptibility of Foeniculum vulgare Seed Essential OilAmerican Journal of Plant Sciences, 2015
- High Performance Liquid Chromatography Method for the Determination of Anethole in Rat PlasmaTropical Journal of Pharmaceutical Research, 2014
- Evaluation Of The Essential Oil Of Foeniculum Vulgare Mill (Fennel) Fruits Extracted By Three Different Extraction Methods By Gc/MsAfrican Journal of Traditional, Complementary and Alternative Medicines, 2014
- Ultrasound-assisted temperature-controlled ionic-liquid dispersive liquid-phase microextraction method for simultaneous determination of anethole, estragole, and para-anisaldehyde in different plant extracts and human urine: a comparative studyAnalytical and Bioanalytical Chemistry, 2014
- Extraction Kinetics and Anethole Content of Fennel (Foeniculum vulgare) and Anise Seed (Pimpinella anisum) Extracts Obtained by Soxhlet, Ultrasound, Percolation, Centrifugation, and Steam DistillationSeparation Science and Technology, 2011
- Chemical Composition, Antimicrobial and Antioxidant Activities of Essential Oils from Organically Cultivated Fennel CultivarsMolecules, 2011
- Determination of anethole in serum samples by headspace solid-phase microextraction-gas chromatography–mass spectrometry for congener analysisJournal of Chromatography A, 2008
- HPLC determination of 4-hydroxy-anethole trithione in plasma via enzymatic hydrolysis and its application to bioequivalence studyJournal of Pharmaceutical and Biomedical Analysis, 2008
- LC Determination of Anethole in Aniseed DrinksChromatographia, 2006
- Identification by HPLC−DAD and HPLC−MS Analyses and Quantification of Constituents of Fennel Teas and DecoctionsJournal of Agricultural and Food Chemistry, 2000