Chromium (VI) bioremoval from contaminated wastewater using Pseudomonas aeruginosa ATHA23 producing biofilm supported on clinoptilolite
- 10 August 2022
- journal article
- research article
- Published by Springer Science and Business Media LLC in Environmental Geochemistry and Health
- Vol. 45 (2), 427-442
- https://doi.org/10.1007/s10653-022-01345-2
Abstract
More has yet to be investigated on the increased efficiency of microbes for the removal of heavy metals from industrial wastewaters. The objective was to determine the Cr (VI) bioabsorption and bioreduction ability of biofilm-producing bacteria supported on clinoptilolite from contaminated aqueous solutions. Chromium (VI)-tolerant bacteria, namely Pseudomonas aeruginosa ATHA23, were identified by biochemical methods and 16S rDNA sequencing and were deposited in NCBI (accession number: KF680991). Preparation of clinoptilolite, bacterial growth and isolation, biofilm production including extracellular polysaccharides (EPS) and Cr (VI) removal efficiency, affected by the experimental treatments, were investigated. The use of FTIR characterized clinoptilolite properties with and without biofilm in the presence and absence of Cr (IV). Higher Cr (VI) levels in the bacterial growth medium, increased EPS production with the highest value (0.171 mg L−1), produced 18 h after treating the bacteria with Cr (VI) (100 mg L−1). However, in the absence of Cr (VI), EPS significantly decreased to 0.117 mg L−1. Plackett–Burman and Taguchi statistical analyses were used to optimize the experimental treatments affecting the removal efficiency of Cr (VI). Among the anions (nitrate, sulfate, and chloride), sulfate decreased Cr removal efficiency. The absorption data were best fitted to the pseudo-second order, and the data of Cr adsorption by clinoptilolite-biofilm were also better fitted to Freundlich isotherm model. The Cr (VI) bioremediation potential of P. aeruginosa ATHA23 by the production of biofilm supported on clinoptilolite has been shown for the first time, which is of significance for the environment and the industry.Keywords
This publication has 45 references indexed in Scilit:
- Characterization of heavy metal (cadmium and nickle) tolerant Gram negative enteric bacteria from polluted Yamuna River, DelhiAfrican Journal of Microbiology Research, 2016
- Bioremediation of chromium solutions and chromium containing wastewatersCritical Reviews in Microbiology, 2014
- Detection of Bacterial 16S rRNA and Identification of Four Clinically Important Bacteria by Real-Time PCRPLOS ONE, 2012
- Removal of nickel(II) ions from aqueous solutions using the natural clinoptilolite and preparation of nano-NiO on the exhausted clinoptiloliteApplied Surface Science, 2010
- Isolation and characterization of ACC deaminase-producing fluorescent pseudomonads, to alleviate salinity stress on canola (Brassica napus L.) growthJournal of Plant Physiology, 2009
- Comparison of methods for total community DNA extraction and purification from compostApplied Microbiology and Biotechnology, 2006
- Specific Extraction of Chromium as Tetrabutylammonium-Chromate and Spectrophotometric Determination by Diphenylcarbazide: Speciation of Chromium in Effluent StreamsAnalytical Sciences, 2003
- Microtiter Plate Assay for Assessment of Listeria monocytogenes Biofilm FormationApplied and Environmental Microbiology, 2002
- Preparation of Genomic DNA from BacteriaCurrent Protocols in Molecular Biology, 2001
- Bacterial reduction of hexavalent chromiumJournal of Industrial Microbiology & Biotechnology, 1995