Quantitative Analysis of Multi-components in Curcuma xanthorrhiza by Single Marker
Open Access
- 31 October 2019
- journal article
- research article
- Published by Universitas Gadjah Mada in Indonesian Journal of Pharmacy
- Vol. 30 (4), 301-308
- https://doi.org/10.14499/indonesianjpharm30iss4pp301
Abstract
A new simple and effective routine analytical method for quantification of curcuminoids in Curcuma xanthorrhiza was developed by high-performance liquid chromatography. This method based on chromatographic fingerprint combined with a quantitative analysis of multi-components by single marker (QAMS). Curcumin was selected as an internal marker for the determination of two other similar compounds, i.e. bisdemethoxycurcumin and demethoxycurcumin, by using the relative coefficient factor (RCF). Excellent linearity was obtained for each component (r2 > 0.9998), and the recovery of extraction methods were within 100.23-103.95%. The precision of the method was good at inter-day and intra-day analysis (RSD < 4.0%). The stability of RCFs was good under various chromatographic conditions with RSD < 1%, and the ratio of retention time was used to locate each compound. The quantification of curcuminoids between QAMS and external standard method (ESM) proved the consistency and similarity of the two method (RSD < 2%). This study demonstrated that QAMS could be used as a routine method for quality control of curcuminoids in C. xanthorrhiza. This method successfully proved accurate, stable, more effective and simple than external standard method.Keywords
This publication has 19 references indexed in Scilit:
- Global Causes of Diarrheal Disease Mortality in Children <5 Years of Age: A Systematic ReviewPLOS ONE, 2013
- Setting Research Priorities to Reduce Mortality and Morbidity of Childhood Diarrhoeal Disease in the Next 15 YearsPLoS Medicine, 2013
- Zinc for the treatment of diarrhoea: effect on diarrhoea morbidity, mortality and incidence of future episodesInternational Journal of Epidemiology, 2010
- Understanding survival analysis: Kaplan-Meier estimateInternational Journal of Ayurveda Research, 2010
- Rotavirus surveillance to determine disease burden and epidemiology in Java, Indonesia, August 2001 through April 2004Vaccine, 2009
- Burden of Severe Rotavirus Diarrhea in IndonesiaThe Journal of Infectious Diseases, 2009
- Zinc and copper supplementation in acute diarrhea in children: a double-blind randomized controlled trialBMC Medicine, 2009
- Zinc supplementation in children with cholera in Bangladesh: randomised controlled trialBMJ, 2008
- Effect of Zinc in Enteropathogenic Escherichia coli InfectionInfection and Immunity, 2007
- Zinc supplementation reduced cost and duration of acute diarrhea in childrenJournal of Clinical Epidemiology, 2007