Development of Multilayered Chlorogenate-Peptide Based Biocomposite Scaffolds for Potential Applications in Ligament Tissue Engineering - An In Vitro Study
- 10 October 2017
- journal article
- Published by Trans Tech Publications, Ltd. in Journal of Biomimetics, Biomaterials and Biomedical Engineering
- Vol. 34, 37-56
- https://doi.org/10.4028/www.scientific.net/jbbbe.34.37
Abstract
In this work, for the first time, chlorogenic acid, a natural phytochemical, was conjugated to a lactoferrin derived antimicrobial peptide sequence RRWQWRMKKLG to develop a self-assembled template. To mimic the components of extracellular matrix, we then incorporated Type I Collagen, followed by a sequence of aggrecan peptide (ATEGQVRVNSIYQDKVSL) onto the self-assembled templates for potential applications in ligament tissue regeneration. Mechanical properties and surface roughness were studied and the scaffolds displayed a Young’s Modulus of 169 MP and an average roughness of 72 nm respectively. Thermal phase changes were studied by DSC analysis. Results showed short endothermic peaks due to water loss and an exothermic peak due to crystallization of the scaffold caused by rearrangement of the components. Biodegradability studies indicated a percent weight loss of 27.5 % over a period of 37 days. Furthermore, the scaffolds were found to adhere to fibroblasts, the main cellular component of ligament tissue. The scaffolds promoted cell proliferation and displayed actin stress fibers indicative of cell motility and attachment. Collagen and proteoglycan synthesis were also promoted, demonstrating increased expression and deposition of collagen and proteoglycans. Additionally, the scaffolds exhibited antimicrobial activity against Staphylococcus epidermis bacteria, which is beneficial for minimizing biofilm formation if potentially used as implants. Thus, we have developed a novel biocomposite that may open new avenues to enhance ligament tissue regeneration.Keywords
This publication has 108 references indexed in Scilit:
- Engineering strategies to recapitulate epithelial morphogenesis within synthetic three-dimensional extracellular matrix with tunable mechanical propertiesPhysical Biology, 2011
- Quantification of Collagen Organization and Extracellular Matrix Factors within the Healing LigamentMicroscopy and Microanalysis, 2011
- Adult bone marrow stromal cell-based tissue-engineered aggrecan exhibits ultrastructure and nanomechanical properties superior to native cartilageOsteoarthritis and Cartilage, 2010
- Anterior Cruciate Ligament Reconstruction Using Achilles Tendon Allograft: an Assessment of Outcome for Patients Age 30 Years and OlderHSS Journal®: The Musculoskeletal Journal of Hospital for Special Surgery, 2010
- Effect of sulfated glycosaminoglycan digestion on the transverse permeability of medial collateral ligamentJournal of Biomechanics, 2010
- Scaffolding in tissue engineering: general approaches and tissue-specific considerationsEuropean Spine Journal, 2008
- Tendon and ligament engineering in the adult organism: mesenchymal stem cells and gene-therapeutic approachesInternational Orthopaedics, 2007
- FTIR Studies of Collagen Model Peptides: Complementary Experimental and Simulation Approaches to Conformation and UnfoldingJournal of the American Chemical Society, 2007
- Stress fibers are generated by two distinct actin assembly mechanisms in motile cellsThe Journal of cell biology, 2006
- Where tendons and ligaments meet bone: attachment sites (‘entheses’) in relation to exercise and/or mechanical loadJournal of Anatomy, 2006