A universal and enzyme-free immunoassay platform for biomarker detection based on gold nanoparticle enumeration with a dark-field microscope

Abstract
We develop a universal and enzyme-free magnetic bead-based sandwich-format immunoassay platform for biomarker detection by combining secondary antibody functionalized AuNPs and automatic AuNP counting.
Funding Information
  • National Natural Science Foundation of China (21475004, 21535006)