Restricting the Dispersal of Recombinant DNA: Design of a Contained Biological Catalyst
- 1 February 1996
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 14 (2), 189-191
- https://doi.org/10.1038/nbt0296-189
Abstract
To restrict horizontal gene spread and, thus, create organisms whose behavior in the field is more predictable, we have combined a mini-Tn5 cloning system that allows stable insertion of foreign genes into the chromosomes of a variety of Gram-negative bacteria with a gene-containment circuit based on the universal lethal-function colicin E3. Use of the system is exemplified by the construction of a microorganism designed to degrade polychlorinated biphenyls, important environmental pollutants. The relevant genotype of the microorganism is subject to a stringent gene-containment mechanism that provides neither an advantage nor a disadvantage to the host cell for survival, but decreases frequencies of productive chromosomal transfer frequencies by at least four orders of magnitude.Keywords
This publication has 12 references indexed in Scilit:
- Universal barrier to lateral spread of specific genes among microorganismsMolecular Microbiology, 1994
- Chromosomal gene capture mediated by the Pseudomonas putida TOL catabolic plasmidJournal of Bacteriology, 1994
- The biphenyl/polychlorinated biphenyl-degradation locus (bph) of Pseudomonas sp. LB400 encodes four additional metabolic enzymesGene, 1994
- [31] Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposonsMethods in Enzymology, 1994
- A DNA module encoding bph genes for the degradation of polychlorinated biphenyls (PCBs)FEMS Microbiology Letters, 1993
- RELEASE OF RECOMBINANT MICROORGANISMSAnnual Review of Microbiology, 1993
- Genetic Exchange in Natural Microbial CommunitiesAdvances in Microbial Ecology, 1992
- The TOL Plasmids: Determinants of the Catabolism of Toluene and the XylenesPublished by Elsevier BV ,1990
- New derivatives of transposon tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in Gram-negative bacteriaGene, 1989
- Colicin E3 and its immunity genesJournal of Molecular Biology, 1985