Biofilm formation and antibiotic resistance in staphylococcus isolated from different objects

Abstract

Staphylococci have a wide range of pathogenic properties, among which stands increased resistance to antibiotics and the ability to form a biofilm. Being divided into coagulase-positive and coagulase-negative, they have different and, at the same time, common biological properties and may be a source of genetic material for each other. The aim of the study was to examine the properties of staphylococci, isolated from various objects (milk, pigs, companion animals, humans), their resistance to antibiotics, the ability to form a biofilm and the presence of genes, responsible for resistance to methicillin and biofilm formation. In this study we used 89 Staphylococci strains. 18 (20.2 %) strains were coagulase-positive, where 2 (2.2 %) strains, isolated from different animals and humans, had a complete set of phenotypic (plasma coagulation, biofilm formation, resistance to oxacillin and benzylpenicillin) and genotypic (mec A, fem B, ica A, ica D) signs of pathogenicity. Coagulase-negative staphylococci, isolated from various animals and humans, had resistance to oxacillin, benzylpenicillin and the ability to form biofilms, and also had the corresponding genes in their structure (mec A, ica AB, ica D) in 3.3 % of cases. Staphylococci, isolated from different animals and humans, were able to form a biofilm and had the appropriate set of genes (ica D, ica AB) in 5.6 % of cases.