Cell-Based Biohybrid Sensor Device for Chemical Source Direction Estimation
Open Access
- 23 January 2021
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Thinking Skills and Creativity
- Vol. 2021, 8907148
- https://doi.org/10.34133/2021/8907148
Abstract
This paper describes a method to estimate the direction from which the signal molecule reaches the sensor by using living cells. In this context, biohybrid sensors that utilize a sophisticated sensing system of cells can potentially offer high levels of chemical-detection sensitivity and selectivity. However, biohybrid-sensor-based chemical-source-direction estimation has not received research attention because the cellular response to chemicals has not been examined in the context of directional information. In our approach, we fabricated a device that can limit the interface between the cell-laden hydrogel and the chemical solution of interest to enhance the time difference over which the chemical solution reaches the cells. Chemical detection by cells that express specific receptors is reflected as the fluorescence of the calcium indicator within the cells. Our device has eight chambers that each house 3D cell-laden collagen hydrogels facing circularly outward. The device also works as a cover to prevent chemicals from permeating the hydrogel from above. In our study, by observing the time course of the fluorescence emission of each chamber, we were able to successfully estimate the chemical-source direction within an error range of 713. Our results suggest that a combination of microstructure devices embedded with living cells can be used to exploit cell functionalities to yield chemical-source directional information.Keywords
Funding Information
- Japan Society for the Promotion of Science (19H05322)
- New Energy and Industrial Technology Development Organization
This publication has 27 references indexed in Scilit:
- Gold-nanoparticle-based biosensors for detection of enzyme activityTrends in Pharmacological Sciences, 2013
- Structure and dynamics of the M3 muscarinic acetylcholine receptorNature, 2012
- Muscarinic and Nicotinic Acetylcholine Receptor Agonists and Allosteric Modulators for the Treatment of SchizophreniaNeuropsychopharmacology, 2011
- A review of monolithic multichannel quartz crystal microbalance: A reviewAnalytica Chimica Acta, 2011
- FRET-based sensors for the human M1-, M3-, and M5-acetylcholine receptorsBioorganic & Medicinal Chemistry, 2011
- Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptorsProceedings of the National Academy of Sciences of the United States of America, 2010
- Advances in Multi-Sensor Data Fusion: Algorithms and ApplicationsSensors, 2009
- Evaluating cell-surface expression and measuring activation of mammalian odorant receptors in heterologous cellsNature Protocols, 2008
- Three-dimensional odor compassIEEE Transactions on Robotics and Automation, 1999
- Development of an odorant sensor using polymer-coated quartz crystals modified with unusual lipidsBiosensors and Bioelectronics, 1998