Effects of CeO2 Nanomaterial on Pseudomonas Aeruginosa Mediated Bronchiectasis Inflammation Response

Abstract

There are many researches on the preparation of rare earth metals (REM) materials and bactericidal materials, but there are few researches on the preparation of REM oxides based on the laser evaporation (LE) method in the field of bactericidal. In this research, ZnO–CeO₂ nanoparticles (NPs) were obtained by the LE method with 100 W single-mode CW CO₂ laser as the light source and cerous carbonate and zinc acetate as the target materials. While physical characterization of the ZnO–CeO₂ NPs, Staphylococcus aureus (SA), Bacillus subtilis var niger (BSVN), and pseudomonas aeruginosa (PAE) were undertaken as test objects to verify the bactericidal performance of the nanomaterial. A PAE mediated inflammation model of bronchiectasis rats was established, and 40 SD rats were screened and rolled into 4 groups: a control (Ctrl) group, a sham group, a model group without nano solution injection (Model group), and a model group injected with nano solution (Model +NPs group). The effects of nanomaterials on inflammation were analyzed. The results suggested that ZnO–CeO₂ NPs were scattered rod particles with uniform morphological distribution. XRD tests revealed that with the increase of ZnO content, its characteristic peaks in the NPs also increased. The preparation of NPs exhibited good bactericidal performance against SA and BSVN, and increasing the concentration of NPs greatly inhibited the activity of PA. By comparing with the model group, the upregulated IL-17 and IL-6 were inhibited (P β were reversed (P <0.01). It meant that the NPs alleviated the inflammation of bronchiectasis mediated by PA, and thus improved the lung function damaged by inflammation.