Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions
Open Access
- 10 May 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 12 (1), 1-18
- https://doi.org/10.1038/s41467-021-22881-y
Abstract
Inter-tissue interaction is fundamental to multicellularity. Although the basement membrane (BM) is located at tissue interfaces, its mode of action in inter-tissue interactions remains poorly understood, mainly because the molecular and structural details of the BM at distinct inter-tissue interfaces remain unclear. By combining quantitative transcriptomics and immunohistochemistry, we systematically identify the cellular origin, molecular identity and tissue distribution of extracellular matrix molecules in mouse hair follicles, and reveal that BM composition and architecture are exquisitely specialized for distinct inter-tissue interactions, including epithelial–fibroblast, epithelial–muscle and epithelial–nerve interactions. The epithelial–fibroblast interface, namely, hair germ–dermal papilla interface, makes asymmetrically organized side-specific heterogeneity in the BM, defined by the newly characterized interface, hook and mesh BMs. One component of these BMs, laminin α5, is required for hair cycle regulation and hair germ–dermal papilla anchoring. Our study highlights the significance of BM heterogeneity in distinct inter-tissue interactions.Funding Information
- MEXT | RIKEN
- MEXT | Japan Society for the Promotion of Science (25122720, 26670533, 20H03706)
- MEXT | JST | Core Research for Evolutional Science and Technology (JPMJCR1926)
- Uehara Memorial Foundation
- Takeda Science Foundation
- Cosmetology Research Foundation
This publication has 72 references indexed in Scilit:
- The Matrisome: In Silico Definition and In Vivo Characterization by Proteomics of Normal and Tumor Extracellular MatricesMolecular & Cellular Proteomics, 2012
- Paracrine TGF-β Signaling Counterbalances BMP-Mediated Repression in Hair Follicle Stem Cell ActivationCell Stem Cell, 2012
- Nerve-Derived Sonic Hedgehog Defines a Niche for Hair Follicle Stem Cells Capable of Becoming Epidermal Stem CellsCell Stem Cell, 2011
- Establishment of conditional reporter mouse lines at ROSA26 locus for live cell imaginggenesis, 2011
- The Basement Membrane of Hair Follicle Stem Cells Is a Muscle Cell NicheCell, 2011
- β-catenin Activity in the Dermal Papilla Regulates Morphogenesis and Regeneration of HairDevelopmental Cell, 2010
- Transcriptome-based systematic identification of extracellular matrix proteinsProceedings of the National Academy of Sciences of the United States of America, 2008
- Hair Follicle Stem Cells Are Specified and Function in Early Skin MorphogenesisCell Stem Cell, 2008
- Complex Hair Cycle Domain Patterns and Regenerative Hair Waves in Living RodentsJournal of Investigative Dermatology, 2008
- Breakdown of the reciprocal stabilization of QBRICK/Frem1, Fras1, and Frem2 at the basement membrane provokes Fraser syndrome-like defectsProceedings of the National Academy of Sciences of the United States of America, 2006