Artemisia extracts Differ from Artemisinin Effects on Human Hepatic CYP450s 2B6 and 3A4 in vitro
Preprint
- 28 June 2022
- preprint
- research article
- Published by Cold Spring Harbor Laboratory
Abstract
Ethnopharmacological relevance: The Chinese medicinal herb, Artemisia annua L., has been used for >2,000 yr as traditional tea infusions to treat a variety of infectious diseases including malaria, and its use is spreading globally (along with A. afra Jacq. ex Willd.) mainly through grassroots efforts.Aim of the study: Artemisinin is more bioavailable delivered from the plant, Artemisia annua L. than the pure drug, but little is known about how delivery via a hot water infusion (tea) alters induction of hepatic CYP2B6 and CYP3A4 that metabolize artemisinin.Materials and Methods: HepaRG cells were treated with 10 μM artemisinin or rifampicin (positive control), and teas (10 g/L) of A. annua SAM, and A. afra SEN and MAL with 1.6, 0.05 and 0 mg/gDW artemisinin in the leaves, respectively; qPCR, and Western blots, were used to measure CYP2B6 and CYP3A4 responses. Enzymatic activity of these P450s was measured using liver microsomes and P450-Glo assays.Results: All teas inhibited activity of CYP2B6 and CYP3A4. Artemisinin and the high artemisinin-containing tea infusion (SAM) induced CYP2B6 and CYP3A4 transcription, but artemisinin-deficient teas, MAL and SEN, did not. Artemisinin increased CYP2B6 and CYP3A4 protein levels, but none of the three teas did, indicating a post-transcription inhibition by all three teas.Conclusions: This study showed that Artemisia teas inhibit activity and artemisinin autoinduction of CYP2B6 and CYP3A4 post transcription, a response likely the effect of other phytochemicals in these teas. Results are important for understanding Artemisia tea posology.Keywords
Other Versions
- Published version: Version Journal of Ethnopharmacology, 298, preprints
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