Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection
Open Access
- 21 October 2020
- Vol. 20 (20), 5952
- https://doi.org/10.3390/s20205952
Abstract
Photoacoustic imaging is attracting a great deal of interest owing to its distinct advantages over other imaging techniques such as fluorescence or magnetic resonance image. The availability of photoacoustic probes for reactive oxygen and nitrogen species (ROS/RNS) could shed light on a plethora of biological processes mediated by these key intermediates. Tetramethylbenzidine (TMB) is a non-toxic and non-mutagenic colorless dye that develops a distinctive blue color upon oxidation. In this work, we have investigated the potential of TMB as an acoustogenic photoacoustic probe for ROS/RNS. Our results indicate that TMB reacts with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce the blue oxidation product, while ROS, such as the superoxide radical anion, sodium peroxide, hydroxyl radical, or peroxynitrite, yield a colorless oxidation product. TMB does not penetrate the Escherichia coli cytoplasm but is capable of detecting singlet oxygen generated in its outer membrane.Keywords
This publication has 72 references indexed in Scilit:
- Peroxynitrite, a Stealthy Biological OxidantOnline Journal of Public Health Informatics, 2013
- Oxidative Stress and Antioxidant DefenseWorld Allergy Organization Journal, 2012
- Photoacoustic emission from fluorescent nanodiamonds enhanced with gold nanoparticlesBiomedical Optics Express, 2012
- Photodynamic therapy of cancer: An updateCA: A Cancer Journal for Clinicians, 2011
- A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and OrganismsPLoS Biology, 2011
- Reactive Species and Antioxidants. Redox Biology Is a Fundamental Theme of Aerobic LifePlant Physiology, 2006
- Terephthalic acid: A dosimeter for the detection of hydroxyl radicals in vitroLife Sciences, 1994
- A Time-Resolved Photoacoustic Method with Pulsed Laser Excitation in the Condensed Phase: The Relation between Signal Intensity and Decay-Rate ConstantBulletin of the Chemical Society of Japan, 1990
- Rose BengalJournal of Photochemistry and Photobiology A: Chemistry, 1989
- Superoxide-promoted oxidation reactions of aniline and N-methylaniline in dimethyl sulfoxideThe Journal of Organic Chemistry, 1985