Lifelong Adaptation of Gastric Cell Proliferation and Mucosa Structure to Early Weaning-Induced Effects
Open Access
- 13 September 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Physiology
Abstract
The gastric mucosa is disturbed when breastfeeding is interrupted, and such early weaning (EW) condition permanently affects the differentiation of zymogenic cells. The aim of the study was to evaluate the immediate and long-term effects of EW on gastric cell proliferation, considering the molecular markers for cell cycle, inflammation, and metaplasia. Overall, we investigated the lifelong adaptation of gastric growth. Wistar rats were divided into suckling-control (S) and EW groups, and gastric samples were collected at 18, 30, and 60 days for morphology, RNA, and protein isolation. Inflammation and metaplasia were not identified, but we observed that EW promptly increased Ki-67-proliferative index (PI) and mucosa thickness (18 days). From 18 to 30 days, PI increased in S rats, whereas it was stable in EW animals, and such developmental change in S made its PI higher than in EW. At 60 days, the PI decreased in S, making the indices similar between groups. Spatially, during development, proliferative cells spread along the gland, whereas, in adults, they concentrate at the isthmus-neck area. EW pushed dividing cells to this compartment (18 days), increased PI at the gland base (60 days), but it did not interfere in expression of cell cycle molecules. At 18 days, EW reduced Tgfβ2, Tgfβ3, and Tgfbr2 and TβRII and p27 levels, which might regulate the proliferative increase at this age. We demonstrated that gastric cell proliferation is immediately upregulated by EW, corroborating previous results, but for the first time, we showed that such increased PI is stable during growth and aging. We suggest that suckling and early weaning might use TGFβs and p27 to trigger different proliferative profiles during life course.Keywords
Funding Information
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
This publication has 58 references indexed in Scilit:
- MAPK Signaling Pathway Regulates p27 Phosphorylation at Threonin 187 as Part of the Mechanism Triggered by Early-Weaning to Induce Cell Proliferation in Rat Gastric MucosaPLOS ONE, 2013
- The origin of pre-neoplastic metaplasia in the stomach: Chief cells emerge from the MistExperimental Cell Research, 2011
- Sox2+ Adult Stem and Progenitor Cells Are Important for Tissue Regeneration and Survival of MiceCell Stem Cell, 2011
- EGFR is involved in control of gastric cell proliferation through activation of MAPK and Src signalling pathways in early-weaned ratsCell Proliferation, 2011
- TFF2 mRNA Transcript Expression Marks a Gland Progenitor Cell of the Gastric Oxyntic MucosaGastroenterology, 2010
- Early weaning accelerates the differentiation of mucous neck cells in rat gastric mucosa: Possible role of TGFα/EGFRDifferentiation, 2010
- Analyzing real-time PCR data by the comparative CT methodNature Protocols, 2008
- A Molecular Signature of Gastric Metaplasia Arising in Response to Acute Parietal Cell LossGastroenterology, 2008
- Ontogenic expression of TGFβ 1, 2, and 3 and its receptors in the rat gastric mucosaDevelopmental Dynamics, 2003
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976