Abstract: | Present study was conducted to investigate the concentration of heavy metals (Na, K, Fe, Zn, Cu, Ni, Mg, Co, Cd and Pb) in different organs (heart, liver, muscles, wings, neck) of poultry, cattle (mutton, beef) and fish available in local and branded markets of Lahore. Atomic Absorption Spectroscopy and Flame Photometry were used to estimate the contamination of these metals in meat. The concentration of heavy metals in different organs showed great variation. The detected values of Na, K, Fe, Mg and Ni were under the tolerable level cited by international standards; World Health Organization (WHO) and Australia New Zealand Food Authority (ANZFA). The Co, Cd and Pd were almost absent in red meat samples but poultry meat wing samples had high concentration of Cu exceeding the permissible limits. Rapid and sensitive Lowry method was adopted for scrutinizing the protein content in internal organs (heart, liver, breast, wings, neck and leg) of poultry meat (Home raised, Local, Zenith and Menu). The results showed that out of all the tested samples liver of Home raised chicken was a rich source of protein. Quantitative evaluation of steroid (testosterone) in chicken through High Performance Liquid Chromatography confirmed that the level of testosterone in different organs didn’t exceed the acceptable limit.