Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells
Open Access
- 15 March 2023
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Pharmacology
- Vol. 14, 1068849
- https://doi.org/10.3389/fphar.2023.1068849
Abstract
Acer truncatum Bunge (Sapindaceae or formerly Aceraceae) is a tall deciduous tree native to China. Traditionally, the leaves of A. truncatum are decocted and used by Chinese Mongolians, Koreans, and Tibetans to treat skin itching, dry cracks, and other skin ailments, which indicates A. truncatum leaves may have a potential inhibitory effect on various skin inflammations. To examine the protective effect against skin inflammations of A. truncatum leaf extract (ATLE), an in vitro dermatitis model was established using sodium dodecyl sulfate (SLS)-induced HaCaT cells. The anti-inflammatory effect of ATLE was evaluated by analyzing cell viability, apoptosis, reactive oxygen species (ROS), interleukin 6 (IL-6), and prostaglandin E2 (PGE2) levels. Orthogonal experiments showed that the pretreatment with ATLE can reduce the IL-6 levels, PGE2 levels, and apoptosis increased in SLS-stimulated HaCaT cells, which indicates that ATLE has positive efficacy for dermatitis. Furthermore, three flavonoid compounds kaempferol-3-O-α-L-rhamnoside, quercetin-3-O-α-L-rhamnopyranoside, kaempferol-3,7-di-O-α-L-rhamnoside, and 1,2,3,4,6-Penta-O-galloyl-β-D-glucopyranose (PGG) were isolated and identified. Among them, kaempferol-3,7-di-O-α-L-rhamnoside was isolated from this plant for the first time. These compounds have been proven to have an anti-inflammatory effect. They may contribute to the efficacy of A. truncatum in treating skin inflammation. The results revealed that ATLE has the potential to be used as an additive in various skin care products to prevent skin inflammations and may be incorporated in formulations for topical application as a therapeutic approach to dermatitis.Funding Information
- National Natural Science Foundation of China
- Minzu University of China
This publication has 77 references indexed in Scilit:
- Prostaglandin E2 and T cells: friends or foes?Immunology & Cell Biology, 2011
- Application of the Aqueous Porous Pathway Model to Quantify the Effect of Sodium Lauryl Sulfate on Ultrasound-Induced Skin Structural PerturbationJournal of Pharmaceutical Sciences, 2011
- Isolation and identification of pentagalloylglucose with broad-spectrum antibacterial activity from Rhus trichocarpa MiquelFood Chemistry, 2010
- Pathogen Recognition and Inflammatory Signaling in Innate Immune DefensesClinical Microbiology Reviews, 2009
- The antibacterial efficacy of an aceraceous plant [Shantung maple (Acer truncatum Bunge)] may be related to inhibition of bacterial β‐oxoacyl‐acyl carrier protein reductase (FabG)Biotechnology and Applied Biochemistry, 2008
- Flavonoids and Xanthones from Tripterospermum chinenseChemistry of Natural Compounds, 2008
- The immune system in healthy adults and patients with atopic dermatitis seems to be affected differently by a probiotic interventionClinical & Experimental Allergy, 2007
- Null Mutations in the Filaggrin Gene (FLG) Determine Major Susceptibility to Early-Onset Atopic Dermatitis that Persists into AdulthoodJournal of Investigative Dermatology, 2007
- Clinical aspects, epidemiology, and prognosis of atopic dermatitisAnnals of Allergy, Asthma & Immunology, 1999
- Surfactant-induced skin irritation and skin repair: Evaluation of a cumulative human irritation model by noninvasive techniquesJournal of the American Academy of Dermatology, 1994