Microscopic structure of pig liver for use in feeding of natural feed additives LG-MAX and Sel-Plex

Abstract

The use of feed additives consisting of organic components and containing fatty acids, namely polyunsaturated fatty acids, of the omega-3 family, is necessary for the regulation of numerous biological processes and support of the body's metabolic homeostasis. The material for this study was young meat-lard pigs (Landrace + Large White) and muscle tissue samples from the longissimus dorsi (m. Longisimus dorsi) pigs taken at the level of 10-12 thoracic vertebrae, at the time of slaughter, at the end of the study period. For histological examination, the selected material was fixed in a 10% formalin solution according to Lilly, embedded in paraffin, and histosections with a thickness of 10 μm were made, which were stained with Karatsi's hematoxylin and eosin. The obtained preparations were examined under a light microscope with a Micros MCQ-2000 photo attachment. The liver of the pigs in the experimental groups did not differ in appearance from the liver of the pigs in the control group. However, according to the histological examination of the liver of the pigs of the experimental group, which were fed the feed additive LG-MAX 2.0 g / day, the presence of single dystrophic processes in hepatocytes was established in the liver of the pigs of the experimental group, which were fed the feed additive LG-MAX 4.0 g / day - the presence of granular and fatty infiltrative dystrophy and vascular hyperemia inside the lobule, and in the liver of pigs that were fed the feed additive LG-MAX 2.0 g / day together with Sel-Plex - in addition, a weakly expressed lymphohistiocytic infiltration of the stroma was established.