CRL4 regulates recombination and synaptonemal complex aggregation in the Caenorhabditis elegans germline

Abstract

To maintain the integrity of the genome, meiotic DNA double strand breaks (DSBs) need to form by the meiosis-specific nuclease Spo11 and be repaired by homologous recombination. One class of products formed by recombination are crossovers, which are required for proper chromosome segregation in the first meiotic division. The synaptonemal complex (SC) is a protein structure that connects homologous chromosomes during meiotic prophase I. The proper assembly of the SC is important for recombination, crossover formation, and the subsequent chromosome segregation. Here we identify the components of Cullin RING E3 ubiquitin ligase 4 (CRL4) that play a role in SC assembly in Caenorhabditis elegans. Mutants of the CRL4 complex (cul-4, ddb-1, and gad-1) show defects in SC assembly manifested in the formation of polycomplexes (PCs), impaired progression of meiotic recombination, and reduction in crossover numbers. PCs that are formed in cul-4 mutants lack the mobile properties of wild type SC, but are likely not a direct target of ubiquitination. In C. elegans, SC assembly does not require recombination and there is no evidence that PC formation is regulated by recombination as well. However, in one cul-4 mutant PC formation is dependent upon early meiotic recombination, indicating that proper assembly of the SC can be diminished by recombination in some scenarios. Lastly, our studies suggest that CUL-4 deregulation leads to transposition of the Tc3 transposable element, and defects in formation of SPO-11-mediated DSBs. Our studies highlight previously unknown functions of CRL4 in C. elegans meiosis and show that CUL-4 likely plays multiple roles in meiosis that are essential for maintaining genome integrity. Defects in the formation of the structure named the synaptonemal complex (SC) lead to the missegregation of chromosomes in the divisions that generate sperm and egg cells. In humans, this chromosome missegregation is associated with infertility and developmental disabilities of the surviving progeny. Abnormal SC structures composed of misfolded and aggregated SC proteins are associated with an inability to properly repair DNA damage and accurately segregate meiotic chromosomes. How SC proteins assemble such that they do not form misfolded protein aggregates is poorly understood. The germlines of nematodes (Caenorhabditis elegans) that lack protein components of the Cullin 4 E3 Ubiquitin ligase complex (CRL4), have defects in the formation of the SC that can be due to misfolding of SC proteins and their aggregation. CRL4 appears to be involved in other germline functions that directly affect chromosome stability (DNA damage repair and transposition), indicating that CRL4 has a central function in the formation of functional sperm and egg cells.