The Effects of Silymarin Supplementation on Post-Thawed Human Sperm Functional Parameters
- 16 November 2022
- journal article
- research article
- Published by Mary Ann Liebert Inc in Biopreservation and Biobanking
- Vol. 21 (6), 569-575
- https://doi.org/10.1089/bio.2022.0065
Abstract
This study investigated the effect of silymarin on human sperm quality during cryopreservation. Samples were collected from 20 normospermic individuals, and each sample was divided into different concentrations of silymarin comprising the following groups: (0, 20, 100, 500, and 1000 μg/mL silymarin). Sperm quality parameters, such as plasma membrane integrity, mitochondrial membrane potential, acrosomal membrane integrity, and caspase 3 were estimated. Silymarin concentrations of 100–500 μg/mL significantly increased motility, plasma membrane integrity, and mitochondrial activity compared with the frozen control group. Acrosomal integrity was increased in the 1000 μg/mL silymarin group. Moreover, 20 and 100 μg/mL concentrations significantly decreased the percentage of caspase 3. The addition of silymarin antioxidant to the frozen medium reduced damage in the sperm after freezing and thawing. This is the first study that showed silymarin can be useful in cryopreservation of human sperm.Keywords
This publication has 33 references indexed in Scilit:
- Maternal Fructose and/or Salt Intake and Reproductive Outcome in the Rat: Effects on Growth, Fertility, Sex Ratio, and Birth Order1Biology of Reproduction, 2013
- Quercetin improves the postthaw characteristics of cryopreserved sex-sorted and nonsorted stallion spermTheriogenology, 2013
- Melatonin as a potential tool against oxidative damage and apoptosis in ejaculated human spermatozoaFertility and Sterility, 2010
- Rhodiola sacra aqueous extract (RSAE) improves biochemical and sperm characteristics in cryopreserved boar semenTheriogenology, 2009
- Interrelationship Between Plasma Membrane Integrity, Mitochondrial Membrane Potential and DNA Fragmentation in Cryopreserved Bovine SpermatozoaReproduction in Domestic Animals, 2008
- Activation of Caspases in Human Spermatozoa during Cryopreservation – An Immunoblot StudyCell and Tissue Banking, 2006
- Detection of damage in mammalian sperm cellsTheriogenology, 2006
- Cryopreservation and Thawing Is Associated with Varying Extent of Activation of Apoptotic Machinery in Subsets of Ejaculated Human Spermatozoa1Biology of Reproduction, 2004
- Comparative evaluation of the effect of antioxidants in the conservation of ram semenActa Veterinaria Hungarica, 2002
- Tissue injury by reactive oxygen species and the protective effects of flavonoidsFundamental & Clinical Pharmacology, 1998