Assessment of Cattle Bull Semen Preservability Using Tris Extender Enriched with Turmeric Extract

Abstract

The objective was to evaluate the effect of tris citric acid fructose egg yolk (TCFY) extender supplemented with turmeric extract on cattle bull semen preservability. Pooled bull semen were extended with Tris extender enriched with 0 µl turmeric extract /5ml (control), 100µl /5ml (TT1), 200 µl /5ml (TT2), 300 µl /5ml (TT3) The semen samples were added to reach a final sperm concentration 60 × 106/ml. Extended semen were subjected to semen freezing protocol. Semen parameters and conception rate were carried out. The post cooling semen characteristics revealed apparent improvement in sperm motility, alive and sperm abnormalities for all concentrations, sperm membrane integrity (HOST) was significantly ameliorated in TT1 and TT2, while acrosome integrity was significantly enhanced in TT1 when compared to the control. The post thawing results exhibited significant improvement in sperm motility for all concentrations and significant amelioration in sperm membrane integrity (HOST) for all concentrations when compared to the control. Sperm abnormalities for all the concentrations and the control treatment were indifferent. Acrosome integrity, in TT1 and TT2, did not significantly differ than the control while TT3 was significantly decreased when compared to the control and the other concentrations. Conception rate was the best in all concentrations if compared to the control. It is concluded that, in cooled and post- thawed semen, the superior semen quality was attained in TT1 and TT2. Conception rate was the best in all concentrations especially inTT3