Construction of Arabidopsis At2g34610 Gene Editing and Overexpression Vector

Abstract

The signal transduction involved in ABA regulates various stages of plant growth and develop-ment, such as seed germination, stomatal closure, root development and senescence, and regu-lates plant adaptation to drought, cold, heat, salt and other stresses. The study of ABA signal transduction pathway is of great significance for understanding the regulation mechanism of plant growth and development. The results of RT-PCR showed that At2g34610 was an ABA-responsive gene and its expression was up-regulated by ABA. In order to study the function of At2g34610, gene multi-target editing vector and overexpression vector were constructed respectively. The vector construction laid a foundation for the creation of target gene overexpression and mutant plants, which are ideal experimental materials for exploring the role and molecular mechanism of At2g34610 in ABA signal transduction and regulation of stress resistance in Arabidopsis thaliana.