Sleep Phases in Crayfish: Relationship Between Brain Electrical Activity and Autonomic Variables

Abstract

In vertebrates like mammals and birds, two types of sleep have been identified: rapid eye movement and non-rapid eye movement sleep. Each one is associated with specific electroencephalogram patterns and is accompanied by variations in cardiac and respiratory frequencies. Sleep has been demonstrated only in a handful of invertebrates, and evidence for different sleep stages remains elusive. Previous results show that crayfish sleeps while lying on one side on the surface of the water, but it is not known if this animal has sleep phases. Heart rate and respiratory frequency are modified by diverse changes in the crayfish environment during wakefulness, and previously, we showed that variations in these variables are present during sleep despite that there are no autonomic anatomical structures described in this animal. Here, we conducted experiments to search for sleep phases in crayfish and the relationships between sleep and cardiorespiratory activity. We used the wavelet transform, grouping analysis with k-means clustering, and principal component analysis, to analyze brain and cardiorespiratory electrical activity. Our results show that (a) crayfish can sleep lying on one side or when it is motionless and (b) the depth of sleep (measured as the power of electroencephalographic activity) changes over time and is accompanied by oscillations in cardiorespiratory signal amplitude and power. Finally, we propose that in crayfish there are at least three phases of sleep.