Objective: To estimate the hematological and biochemical reference values inMAteles geoffroyi individuals in captivity. Methodology: Eleven males and 23 females were captured. We collected blood,from the coccygian vein; conventional techniques were used to analyze thesamples.Results: mean corpuscular volume (MCV; P < 0.03), platelets (PLT; P < 0.04) were significantly higher in females than in males. In relation to biochemical blood values wasn’t significantly in male and female groups.Limitations/Implications: hematological and biochemical reference values of A. geoffroyi are within normal health parameters, are between the ranges reported for other species of neotropical primates and can be used as a reference in for the management of the health of this species in captivity.Conclusions: hematological and biochemical parameters of captive specimens of A. geoffroyi are described, which provide reference indicators for the management of the health of the species.