Simultaneous determination of carbinoxamine maleate and pseudoephedrine HCl in their pure form and in their pharmaceutical formulation by HPTLC-densitometric method
Open Access
- 31 March 2016
- journal article
- Published by European Journal of Chemistry in European Journal of Chemistry
- Vol. 7 (1), 37-41
- https://doi.org/10.5155/eurjchem.7.1.37-41.1355
Abstract
An accurate, sensitive and selective High Performance Thin Layer Chromatography (HPTLC) method has been developed and validated for determination of carbinoxamine maleate and pseudoephedrine HCl in their binary mixture without previous separation. Chromatographic separation was performed on aluminum plates precoated with silica gel 60F254 using acetone: methanol:ethylacetate:triethylamine (5:4:2:0.2, v:v:v:v) as a developing system. The bands were scanned at 215 nm. The proposed method has been validated as per ICH guidelines and its linearity was evident in the ranges of 0.4-8.0 and 4-24 µg/ band for carbinoxamine maleate and pseudoephedrine HCl, respectively. The developed method has been applied for determination of the above mentioned drugs in their pharmaceutical formulation where no interference from methyl paraben present as excipient has been detected. Statistical comparison of the results obtained by the developed HPTLC method and those obtained by the reported HPLC method showed no significant difference between them. The developed method is sensitive, accurate and precise and can be easily used for quality control analysis of the studied drugs.Keywords
This publication has 16 references indexed in Scilit:
- Stability-Indicating TLC–Densitometric Method for Simultaneous Determination of Paracetamol and Chlorzoxazone and their Toxic ImpuritiesJournal of Chromatographic Science, 2012
- Simultaneous spectrophotometric and liquid chromatographic determination of dextromethorphan hydrobromide, phenylephrine hydrochloride, and carbinoxamine maleate in pharmaceutical preparationsJournal of Analytical Chemistry, 2011
- Simultaneous determination of pseudoephdrine, pheniramine, guaifenisin, pyrilamine, chlorpheniramine and dextromethorphan in cough and cold medicines by high performance liquid chromatographyTalanta, 2009
- HPTLC-densitometric analysis of candesartan cilexetil and hydrochlorothiazide in tabletsJournal of Planar Chromatography – Modern TLC, 2008
- A new hydrophilic interaction liquid chromatographic (HILIC) procedure for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold formulationsJournal of Pharmaceutical and Biomedical Analysis, 2007
- Simultaneous analysis of the H1-antihistamine acrivastine and the decongestant pseudoephedrine hydrochloride by high-performance liquid chromatographyJournal of Pharmaceutical and Biomedical Analysis, 2005
- A Quantitative Simultaneous High Performance Liquid Chromatographic Determination of Pseudoephedrine HCl, Guaifenesin and Dextromethorphan HBr.Analytical Letters, 1997
- Spectrophotometric Determination of the Antihistamines, Carbinoxamine Maleate and Doxylamine Succinate, in anN-Cetylpyridinium Chloride Micellar MediumAnalytical Letters, 1996
- The Use of First-Order UV Spectroscopy to Determine Binary Mixtures of Pharmaceuticals (A) PHEH and CBOM and (B) DPHH and NPZHAnalytical Letters, 1991
- Spectrophotometric determination of carbinoxamine maleate in tablets containing caffeine, sulphamethoxypyridazine and alginic acidThe Analyst, 1968