Taxus cuspidata Sieb. ET. Zucc. is a taxus of Taxaceae, a rare third-order relict species distributed in northeastern China, and a wild endangered plant species protected by national level I. Taxol (paclitaxel, trade name taxol) and cephalomannine (cephalomannine) are all diterpenoids contained in the genus Taxus, with broad-spectrum anti-tumor activity and unique anti-cancer mechanism. In this study, the distribution of paclitaxel and cephalomannine in the leaves of Taxus cuspidata in different parts and different growth stages was discussed. The results showed that the content of two substances in the leaves of the majority of the crowns was lower than that of the biennial and tertiary there were no significant differences in the contents of two substances in the two-year and three-year-old foliage. There was no significant difference in the contents of the two layers in the three levels of the noodles, and the content of the male was slightly higher than that of the dark. The content of paclitaxel in the leaves of natural northeast yew was the highest at dormancy period, and the content of flowering and fruit was not much different. The content of Cephalotaxin was the highest in dormancy period, and that of cephalosporin the content of paclitaxel and cephalomannine in each plant were significantly different. There was significant difference between the two plants.