Sığırlardan İzole Edilen Pasteurella multocida Suşlarında Çeşitli Antimikrobiyal Maddelerin MİK Değerlerinin ve Antimikrobiyal Direnç Genlerinin Belirlenmesi
- 1 January 2021
- journal article
- research article
- Published by Kafkas University in Kafkas Universitesi Veteriner Fakultesi Dergisi
- Vol. 27 (2), 217-224
- https://doi.org/10.9775/kvfd.2020.25093
Abstract
Pasteurella multocida is an important bacterium that can cause respiratory infections in cattle. Due to the usage of antimicrobial agents in the treatment of the disease frequently, it is critical to follow the antimicrobial susceptibility of the isolates. In this study, minimal inhibitory concentrations (MIC) of various antimicrobial agents and presence of genes related to resistance were investigated in 59 P. multocida strains isolated from the respiratory tract of cattle. According to MIC values determined by E-test, all of the isolates were susceptible to enrofloxacin, chloramphenicol and gentamicin, but resistant to cefoxitin. In addition, high resistance to ampicillin (88.14%), tilmicosin (64.41%), clindamycin (83.05%) and streptomycin (59.32%) were observed in the isolates. When the resistance genes were examined by PCR, it was determined that blaROB-1, tet H, sul II, str A/aphA 1 and erm 42 genes could play an important role in penicillin, tetracycline, sulfamethoxazole + trimethoprime, aminoglycoside and macrolide resistance, respectively. It was concluded that the usage of ampicillin, tetracycline, sulfamethoxazole + trimethoprime, macrolide and aminoglycosides should be considered for the treatment of respiratory tract infections caused by P. multocida in cattle. Also, it was determined that antimicrobial resistance genes could play an important role in the development of resistance in P. multocida.Keywords
This publication has 23 references indexed in Scilit:
- Methodological comparisons for antimicrobial resistance surveillance in feedlot cattleBMC Veterinary Research, 2013
- Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocidaAntimicrobial Agents and Chemotherapy, 2012
- ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: analysis of the regions that comprise 12 antimicrobial resistance genesJournal of Antimicrobial Chemotherapy, 2011
- Combinations of Macrolide Resistance Determinants in Field Isolates of Mannheimia haemolytica and Pasteurella multocidaAntimicrobial Agents and Chemotherapy, 2011
- Molecular Basis of Macrolide, Triamilide, and Lincosamide Resistance in Pasteurella multocida from Bovine Respiratory DiseaseAntimicrobial Agents and Chemotherapy, 2011
- Diagnostic and typing options for investigating diseases associated with Pasteurella multocidaVeterinary Microbiology, 2008
- Antimicrobial resistance profile of Actinobacillus pleuropneumoniae and Actinobacillus porcitonsillarumVeterinary Microbiology, 2007
- Distribution of Tetracycline Resistance Genes in Actinobacillus pleuropneumoniae Isolates from SpainAntimicrobial Agents and Chemotherapy, 2006
- Novel Spectinomycin/Streptomycin Resistance Gene, aadA14 , from Pasteurella multocidaAntimicrobial Agents and Chemotherapy, 2005
- Antimicrobial Resistance Genes in Enterotoxigenic Escherichia coli O149:K91 Isolates Obtained over a 23-Year Period from PigsAntimicrobial Agents and Chemotherapy, 2003