Canary in the coliform mine: Exploring the industrial application limits of a microbial respiration alarm system
Open Access
- 4 March 2021
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 16 (3), e0247910
- https://doi.org/10.1371/journal.pone.0247910
Abstract
Fundamental ecological principles of ecosystem-level respiration are extensively applied in greenhouse gas and elemental cycle studies. A laboratory system termed CEMS (Carbon Dioxide Evolution Measurement System), developed to explore microbial biofilm growth and metabolic responses, was evaluated as an early-warning system for microbial disturbances in industrial settings: in (a) potable water system contamination, and (b) bioreactor inhibition. Respiration was detected as CO2 production, rather than O2 consumption, including aerobic and anaerobic metabolism. Design, thresholds, and benefits of the remote CO2 monitoring technology were described. Headspace CO2 correlated with contamination levels, as well as chemical (R2 > 0.83–0.96) and microbiological water quality indicators (R2 > 0.78–0.88). Detection thresholds were limiting factors in monitoring drinking water to national and international standards (0 CFU/100 mL fecal coliforms) in both open- (>1500 CFU/mL) and closed-loop CO2 measuring regimes (>100 CFU/100 mL). However, closed-loop detection thresholds allow for the detection of significant contamination events, and monitoring less stringent systems such as irrigation water (2 fluctuations from chemical CO2 dynamics, to optimize this real-time, sustainable, low-waste technology, facilitating timeous responses to biological disturbances in bioreactors.Other Versions
Funding Information
- Horizon 2020 (689925)
This publication has 20 references indexed in Scilit:
- Tracking the cellulolytic activity of Clostridium thermocellum biofilmsBiotechnology for Biofuels, 2013
- Increase in observed net carbon dioxide uptake by land and oceans during the past 50 yearsNature, 2012
- Comparison of Fluorescent Marker Systems with 2 Quantitative Methods of Assessing Terminal Cleaning PracticesInfection Control & Hospital Epidemiology, 2011
- Biofilm form and function: carbon availability affects biofilm architecture, metabolic activity and planktonic cell yieldJournal of Applied Microbiology, 2010
- Pronounced Effect of the Nature of the Inoculum on Biofilm Development in Flow SystemsApplied and Environmental Microbiology, 2010
- CO 2 Production as an Indicator of Biofilm MetabolismApplied and Environmental Microbiology, 2009
- Biofilm problems in dental unit water systems and its practical controlJournal of Applied Microbiology, 2009
- Detection and Typing of Campylobacter jejuni and Campylobacter coli and Analysis of Indicator Organisms in Three Waterborne Outbreaks in FinlandApplied and Environmental Microbiology, 2003
- Waterborne Outbreak of Norwalk-Like Virus Gastroenteritis at a Tourist Resort, ItalyEmerging Infectious Diseases, 2002
- On the Temperature Dependence of Soil RespirationFunctional Ecology, 1994