The Effect of Sucrose Supplementation on the Micropropagation of Salix viminalis L. Shoots in Semisolid Medium and Temporary Immersion Bioreactors
Open Access
- 15 October 2021
- Vol. 12 (10), 1408
- https://doi.org/10.3390/f12101408
Abstract
The effect of sucrose concentration on the micropropagation of axillary shoots of willow was investigated. The following factors were examined: the culture system (semisolid medium in glass jars versus liquid medium in temporary immersion bioreactors), the type of explant (apical and basal sections), the frequency of immersion, and CO2 enrichment. Shoots and leaf growth were significantly higher in RITA® bioreactors than in the jars for all the sucrose treatments. Apical or basal sections of willow cultured in bioreactors under high light intensity (150 µmol m−2 s−1) and ventilated six times a day with CO2-enriched air were successfully proliferated without sucrose, whereas shoots cultured in jars did not proliferate well if sucrose concentration was 0.5% or lower. More roots were formed when sucrose was added to the medium. Shoots cultured in bioreactors were successfully acclimatized irrespective of the sucrose treatment and the root biomass when transferred to ex vitro conditions. This is the first report of photoautotrophic willow micropropagation, our results confirm the importance of proper gaseous exchange to attain autotrophy during in vitro propagation.Keywords
Funding Information
- Xunta de Galicia (IN607A, Contrato Programa 2019-2020)
- CYTED Ciencia y Tecnología para el Desarrollo (P117RT0522)
This publication has 55 references indexed in Scilit:
- Effect of different carbon sources on the accumulation of carbohydrate, nutrient absorption and the survival rate of Chinese Ash (Fraxinus mandshurica) explants in vitroAfrican Journal of Agricultural Research, 2012
- Development and application of photoautotrophic micropropagation plant systemPlant Cell, Tissue and Organ Culture (PCTOC), 2010
- A neurofuzzy logic approach for modeling plant processes: A practical case of in vitro direct rooting and acclimatization of Vitis vinifera L.Plant Science, 2010
- Biomass productivity and phytoremediation potential of Salix alba and Salix viminalisBiomass and Bioenergy, 2010
- Leaf ultrastructure, photosynthetic rate and growth of myrtle plantlets under different in vitro culture conditionsBiologia plantarum, 2006
- Effects of CO2 and sugars on photosynthesis and composition of avocado leaves grown in vitroPlant Physiology and Biochemistry, 1999
- Photosynthesis and carbon metabolism in leaves formed prior and during ex vitro acclimatization of micropropagated plantsPlant Science, 1998
- The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different ResolutionJournal of Plant Physiology, 1994
- How Important is Photosynthesis in Micropropagation?Published by Springer Science and Business Media LLC ,1990
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962