Strengthening of Molecular Diagnosis of SARS-CoV-2 / COVID-19 with a Special Focus on India
Open Access
- 14 May 2020
- journal article
- review article
- Published by Journal of Pure and Applied Microbiology in Journal of Pure and Applied Microbiology
- Vol. 14 (suppl 1), 789-798
- https://doi.org/10.22207/jpam.14.spl1.16
Abstract
Severe acute respiratory syndrome corona virus-2 (SARS-CoV-2), a novel coronavirus initially reported in Wuhan, China, is the causative agent of coronavirus disease (COVID-19) pandemic. Symptoms of the disease comprise of fever, tiredness, dry cough, aches and pains, nasal congestion, runny nose, sore throat, diarrhoea and pneumonia at the late stage. SARS-CoV-2 has severely crippled the healthcare system and has caused huge economic losses. Following the outbreak, the SARS-CoV-2 was recognized timely and its genome was sequenced, leading to the development of real-time polymerase chain reaction assays for its detection in clinical samples collected from suspected cases. The management of the pandemic is limited by a number of misconceptions and insufficient information about laboratory testing for SARS-CoV-2 to confirm the disease. This includes a lack of awareness about procedures for the collection, transport, testing, and handling of biological samples for COVID diagnosis. This article provides an overview of the current laboratory diagnostic methods with a purpose to provide information and guidance to laboratories, stakeholders, broader community and especially public health professionals involved in laboratory testing for SARS-CoV-2.Keywords
This publication has 35 references indexed in Scilit:
- Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus–Infected PneumoniaThe New England Journal of Medicine, 2020
- Transmission of 2019-nCoV Infection from an Asymptomatic Contact in GermanyThe New England Journal of Medicine, 2020
- Pattern of early human-to-human transmission of Wuhan 2019 novel coronavirus (2019-nCoV), December 2019 to January 2020Eurosurveillance, 2020
- Note from the editors: novel coronavirus (2019-nCoV)Eurosurveillance, 2020
- Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat originPublished by Cold Spring Harbor Laboratory ,2020
- Outbreak of pneumonia of unknown etiology in Wuhan, China: The mystery and the miracleJournal of Medical Virology, 2020
- MERS, SARS and other coronaviruses as causes of pneumoniaRespirology, 2017
- Serological assays for emerging coronaviruses: Challenges and pitfallsVirus Research, 2014
- Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrationsNature Biotechnology, 2010
- A novel magnetic bead bioassay platform using a microchip-based sensor for infectious disease diagnosisJournal of Immunological Methods, 2006